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. 1988 Nov;45(3):416-7.

Use of a monoclonal antibody in the diagnosis of infection by Dermatophilus congolensis

S J How  1 , D H Lloyd, J Lida
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  • PMID: 3062721

Use of a monoclonal antibody in the diagnosis of infection by Dermatophilus congolensis

S J How et al. Res Vet Sci. 1988 Nov.

Abstract

A monoclonal antibody (McAb) to Dermatophilus congolensis was produced from murine hybridoma cultures and purified by affinity chromatography. Species specificity was demonstrated using indirect immunofluorescent staining; the McAb was shown to react with 10 D congolensis isolates but not with 10 Nocardia species isolates, a Rhodococcus and a Streptomyces species isolate. The McAb was used to demonstrate D congolensis in clinical material from confirmed bovine and ovine cases and presumptive equine cases of dermatophilosis by indirect immunofluorescent staining.

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