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. 2019 Feb;46(1):1157-1165.
doi: 10.1007/s11033-018-04582-w. Epub 2019 Jan 9.

Immunomodulatory effect of mesenchymal stem cells: Cell origin and cell quality variations

Marwa El-Sayed  1   2 , Mohamed Ali El-Feky  3   4 , Mostafa I El-Amir  3 , Al Shaimaa Hasan  5   6 , Mohammed Tag-Adeen  7   8 , Yoshishige Urata  5 , Shinji Goto  5 , Lan Luo  5 , Chen Yan  5 , Tao-Sheng Li  5
Affiliations

Immunomodulatory effect of mesenchymal stem cells: Cell origin and cell quality variations

Marwa El-Sayed et al. Mol Biol Rep. 2019 Feb.

Abstract

The immunomodulatory property of mesenchymal stem cells (MSCs) has been previously reported. Still it is unclear if this property can be affected by the cell origin and cell quality. Using primary MSCs expanded from bone marrow (BM-MSCs) and adipose tissue (AD-MSCs) of mice, we investigated whether the immunomodulatory property of MSCs varied with cell origin and cell quality (early- vs. late-passaged BM-MSCs). BM-MSCs (p1) and AD-MSCs (p1) had a typical spindle shape, but morphological changes were observed in late-passaged BM-MSCs (p6). A pathway-focused array showed that the expression of chemokine/cytokine genes varied with different cell origins and qualities. By co-culturing with spleen mononuclear cells (MNC) for 3 days, the expression of CD4 was suppressed by all types of MSCs. By contrast, the expression of CD8 was suppressed by BM-MSCs and increased by AD-MSCs. The expression ratio of CD206 to CD86 was at a comparable level after co-culture with AD-MSCs and BM-MSCs, but was lower with late-passaged BM-MSCs. AD-MSCs highly induced the release of IL6, IL-10 and TGF-β in culture medium. Compared with early-passaged BM-MSCs (p1), late-passaged BM-MSCs (p6) released less TGF-β. Our data suggests that the immunomodulatory properties of MSCs vary with cell origin and cell quality and that BM-MSCs of good quality are likely the optimal source of immunomodulation.

Keywords: AD-MSC; BM-MSC; Immunomodulation; Macrophage; Mesenchymal stem cell; T cell.

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References

    1. Friedenstein AJ, Chailakhjan RK, Lalykina KS (1970) The development of fibroblast colonies in monolayer cultures of guina-pig bone marrow and spleen cells. Cell Prolif 3:393–403 - DOI
    1. Pittenger MF (1999) Multilineage potential of adult human mesenchymal stem cells. Science 284:143–147 - DOI - PubMed
    1. Gao F, Chiu SM, Motan DA et al (2016) Mesenchymal stem cells and immunomodulation: status and prospects. Cell Death Dis. https://doi.org/10.1038/cddis.2015.327 - DOI - PubMed - PMC
    1. Abreu SC, Antunes MA, Xisto DG et al (2017) Bone marrow, adipose, and lung tissue-derived murine mesenchymal stromal cells release different mediators and differentially affect airway and lung parenchyma in experimental asthma. Stem Cells Transl Med. https://doi.org/10.1002/sctm.16-0398 - DOI - PubMed - PMC
    1. Wen L, Zhu M, Madigan MC et al (2014) Immunomodulatory effects of bone marrow-derived mesenchymal stem cells on pro-inflammatory cytokine-stimulated human corneal epithelial cells. PLoS ONE. https://doi.org/10.1371/journal.pone.0101841 - DOI - PubMed - PMC

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