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. 2017 Jun;15(1):87-94.
doi: 10.1016/j.jgeb.2017.02.004. Epub 2017 Mar 23.

Isolation and characterization of bioactive metabolites producing marine Streptomyces parvulus strain sankarensis-A10

Mobeen Shaik  1 , G Girija Sankar  1 , M Iswarya  1 , P Rajitha  1
Affiliations

Isolation and characterization of bioactive metabolites producing marine Streptomyces parvulus strain sankarensis-A10

Mobeen Shaik et al. J Genet Eng Biotechnol. 2017 Jun.

Abstract

The significance and frequency of marine microorganisms as producers of bioactive metabolites-a natural source of drug discovery had varied significantly during the last decades, making marine ecosystem a huge treasure trove of novel isolates and novel compounds. Among the twelve actinomycetes isolated from marine sediment sample (Lat. 17°41'962″N, Long. 83°19'633″E), amylase, protease, lipase and cellulase activities were exhibited by 8,7,4,3 isolates respectively. Five isolates exhibited l-asparaginase activity, while 5, 6, 2 isolates exhibited antibacterial, antifungal and antimicrobial activities respectively. One isolate VMS-A10 efficiently producing alpha-amylase (25.53 ± 0.50 U/mL), protease (19.26 ± 0.25 U/mL), lipase (36.25 ± 0.10 U/mL), cellulase (14.43 ± 0.513 U/mL), l-asparaginase (0.125 ± 0.004 U/mL), antimicrobial metabolites against B. subtilis (503.33 ± 5.77 U/mL), S. aureus (536.66 ± 5.77 U/mL), E. coli (533.33 ± 5.77 U/mL), P. aeruginosa (500.00 ± 10.0 U/mL), MRSA (538.33 ± 5.77 U/mL), C. albicans (353.33 ± 11.54 U/mL) and A. niger (443.33 ± 15.27 U/mL) was selected, identified on the basis of morphological, cultural, physiological, and biochemical properties together with 16S rDNA sequence, designated as Streptomyces parvulus strain sankarensis-A10 and sequencing product (1490 bp) was deposited in the GenBank database under accession number KT906299, Culture Deposit No: NCIM-5601. Isolation and characterization of each potential actinobacteria having immense industrial and therapeutic value on an unprecedented scale from marine sediments of Visakhapatnam coast will have a burgeoning effect.

Keywords: Actinomycetes; Bioactive metabolites; Enzyme assays; Phenotypic and genotypic characterization; Streptomyces.

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Figures

Figure 1
Figure 1
(A) Antibacterial activity (cross streak method). (B) Spore chain morphology of isolate VMS-A10 under 400× magnification (right).
Figure 2
Figure 2
Bioactive metabolites produced by isolate VMS-A10 indicating (A) Alpha amylase. (B) Lipase. (C) l-Asparaginase. (D) Casein hydrolysis. (E) Cellulase. (F) Antibacterial activities.
Figure 3
Figure 3
Scanning electron micrograph of VMS-A10.
Figure 4
Figure 4
Phylogenetic tree using neighbor-joining method for the isolate VMS-A10.
See this image and copyright information in PMC

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