Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1988 Aug;40(1):35-41.
doi: 10.1016/0885-4505(88)90101-6.

Underestimation of D-glucose phosphorylation as measured by 3H2O production from D-[2-3H]glucose

W J Malaisse  1 , M T Yilmaz, F Malaisse-Lagae, A Sener
Affiliations

Underestimation of D-glucose phosphorylation as measured by 3H2O production from D-[2-3H]glucose

W J Malaisse et al. Biochem Med Metab Biol. 1988 Aug.

Abstract

In rat pancreatic islets, tumoral islet cells (RINm5F line), parotid gland, and in human erythrocytes, but not in rat hepatocytes, the production of 3H2O from D-[2-3H]glucose is 20-30% lower than from D-[5-3H]glucose. This coincides with the production of tritiated lactic acid from D-[2-3H]glucose and may be attributable to an intramolecular hydrogen transfer in the phosphoglucoisomerase reaction. It is concluded that the production of 3H2O from D-[2-3H]glucose is not a reliable tool to assess the total rate of hexose phosphorylation.

PubMed Disclaimer

Publication types

LinkOut - more resources