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. 2018 Sep-Dec;22(3):446.
doi: 10.4103/jomfp.JOMFP_271_17.

Immunohistochemical expression of osteonectin, matrix metalloproteinases-9 and Ki-67 in ameloblastoma

K Indirapriyadarsini  1 , Vandana Raghunath  2 , B Venu Naidu  3 , Botu Badari Ramakrishna  4 , Archana Tangudu  5 , K V Lokesh  4
Affiliations

Immunohistochemical expression of osteonectin, matrix metalloproteinases-9 and Ki-67 in ameloblastoma

K Indirapriyadarsini et al. J Oral Maxillofac Pathol. 2018 Sep-Dec.

Abstract

Background: Ameloblastoma (AM) is a benign odontogenic neoplasm characterized by local invasiveness and recurrence. We have evaluated the immunohistochemical expressions of osteonectin (ON), matrix metalloproteinase-9 (MMP-9) and Ki-67 in AM to understand the biologic behavior of this neoplasm.

Aim: The aim of this study is to evaluate the expression of ON, MMP-9 and Ki-67 in AM.

Materials and methods: The study sample included archival tissues embedded in paraffin blocks histopathologically diagnosed as AM (20 cases). Three serial sections of each tissue specimen were stained separately with ON, MMP-9 and Ki-67 (immunohistochemistry stain) and evaluated. The data were analyzed using Spearman rank correlation test.

Results: All AM cases 20/20 (100%) exhibited positive immunostaining for ON, MMP-9 and Ki-67.

Conclusion: Increased expression of ON, MMP-9 and Ki-67 in AM indicates that these molecules might play a role in the regulation and aggressive nature of this neoplasm.

Keywords: Ameloblastoma; Ki-67; matrix metalloproteinase-9; osteonectin.

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Conflict of interest statement

There are no conflicts of interest.

Figures

Figure 1
Figure 1
Osteonectin – Photomicrograph showing (a) weak staining, (b) moderate staining, (c) strong staining, (d) very strong staining of the tumor cells and the subjacent stromal component (×40)
Figure 2
Figure 2
Matrix metalloproteinase-9 – Photomicrograph showing (a) weak staining, (b) moderate staining, (c) strong staining, (d) very strong staining of the tumor cells and the subjacent stromal component (×40)
Figure 3
Figure 3
Ki-67 – Photomicrograph showing strong staining in peripheral columnar as well as parabasal cells (a) ×20 (b) ×40
Graph 1
Graph 1
Percentage of positive and negative immunostaining of osteonectin, matrix metalloproteinase-9 and Ki-67 in 20 cases of ameloblastoma
Graph 2
Graph 2
Total number and percentage distribution of cases based on the immunohistochemistry expression of osteonectin, matrix metalloproteinase-9 and Ki-67 staining in the tumor component of ameloblastoma
See this image and copyright information in PMC

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