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. 2019 Jan;17(1):63-68.
doi: 10.3892/ol.2018.9581. Epub 2018 Oct 16.

Expression pattern and level of ING5 protein in normal and cancer tissues

Xue-Feng Yang  1 , Dao-Fu Shen  1 , Shuang Zhao  1 , Tian-Ren Ren  2 , Yang Gao  1 , Shuai Shi  1 , Ji-Cheng Wu  1 , Hong-Zhi Sun  1 , Hua-Chuan Zheng  1   3
Affiliations

Expression pattern and level of ING5 protein in normal and cancer tissues

Xue-Feng Yang et al. Oncol Lett. 2019 Jan.

Abstract

Inhibitor of growth family 5 (ING5) functions as a type-II tumor suppressor gene and exerts an important role in DNA repair, apoptotic induction, proliferative inhibition, chromatin remodeling and the invasion process. In the present study, immunohistochemistry was performed to characterize the expression profile of ING5 protein on a tissue microarray containing mouse and human normal tissues, and human cancer tissues, including hepatocellular (n=62), renal clear cell (n=62), pancreatic (n=62), esophageal squamous cell (n=45), cervical squamous cell (n=31), breast (n=144), gastric (n=196), colorectal (n=96), endometrial (n=96) and lung carcinoma (n=192). In the mouse tissues, ING5 expression was detected in the cytoplasm of neurons, the nephric tubule and glomerulus, alveolar epithelium, gastrointestinal glands, squamous epithelium of the skin and skeletal muscles. By contrast, ING5 was localized to the cell nucleus in breast tissues. In human tissues, ING5 protein was primarily localized in the cytoplasm. However, ING5 was detected in the cytoplasm and nucleus in various types of normal tissues, including the tongue, stomach, intestine, lung and breast. In total, ING5 expression was detected in 400/986 cancer tissues (40.6%). In the majority of cases, ING5 expression was observed to be restricted to the cytoplasm. However, ING5 was also detected in the nucleus in a number of cancer tissues, including gastric, colorectal and lung carcinoma. Notably, ING5 was more frequently expressed in breast (79.9%), colorectal (56.3%) and endometrial carcinoma (50.0%). The incidence of ING5 expression in hepatocellular carcinoma (14.5%) and pancreatic carcinoma (22.6%) was low. These findings indicate that ING5 may be involved in cell regeneration and be associated with colorectal carcinogenesis.

Keywords: cancer; expression profile; human; immunohistochemistry; inhibitor of growth family 5; mouse.

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Figures

Figure 1.
Figure 1.
Schematic diagram of the human and mouse isoforms of ING5. aa, amino acid; ING5, inhibitor of growth family 5; LZL, leucine zipper like; NCR, novel conserved region; NLS, nuclear localization signal; PHD, plant homeodomain.
Figure 2.
Figure 2.
ING5 protein expression in normal mouse tissues (magnification, ×200). (A) Stomach, (B) intestine, (C) kidney, (D) brain, (E) kidney, (F) breast, (G) heart, (H) lungs and bronchial tissues. ING5, inhibitor of growth family 5.
Figure 3.
Figure 3.
Immunostaining of the ING5 protein in normal human tissues (magnification, ×200). (A) Stomach, (B) intestine, (C) bronchus, (D) lungs, (E) endometrium, (F) breast and (G) pancreatic tissues. ING5, inhibitor of growth family 5.
Figure 4.
Figure 4.
ING5 expression in human cancer issues (magnification, ×200). (A) Gastric, (B) colorectal, (C) esophageal, (D) renal, (E) breast, (F) hepatocellular, (G) pancreatic, (H) lung, (I) endometrial and (J) cervical carcinoma. ING5, inhibitor of growth family 5.
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