. 2019 Mar 15:208:22-31.

doi: 10.1016/j.carbpol.2018.12.035. Epub 2018 Dec 13.

PI3KC3-dependent autophagosomes formation pathway is of crucial importance to anti-DHAV activity of Chrysanthemum indicum polysaccharide

Ke Ming¹, Wenjuan Yuan², Yun Chen³, Hongxu Du⁴, Miao He⁵, Yuanliang Hu⁶, Deyun Wang⁷, Yi Wu⁸, Jiaguo Liu⁹

Affiliations

¹ Institute of Traditional Chinese Veterinary Medicine and MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, PR China. Electronic address: 2017207037@njau.edu.cn.
² Institute of Traditional Chinese Veterinary Medicine and MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, PR China. Electronic address: 2016107097@njau.edu.cn.
³ College of Animal Science and Technology, College of Tropical Agriculture and Forestry, Hainan University, Hainan Key Lab of Tropical Animal Reproduction & Breeding and Epidemic Disease Research, Animal Genetic Engineering Key Lab of Haikou, Haikou, 570228, PR China. Electronic address: ychen@hainu.edu.cn.
⁴ Institute of Traditional Chinese Veterinary Medicine and MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, PR China. Electronic address: 2016207035@njau.edu.cn.
⁵ Institute of Traditional Chinese Veterinary Medicine and MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, PR China. Electronic address: 2017107099@njau.edu.cn.
⁶ Institute of Traditional Chinese Veterinary Medicine and MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, PR China. Electronic address: ylhu@njau.edu.cn.
⁷ Institute of Traditional Chinese Veterinary Medicine and MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, PR China. Electronic address: dywang@njau.edu.cn.
⁸ Institute of Traditional Chinese Veterinary Medicine and MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, PR China. Electronic address: wuyi2001cn@163.com.
⁹ Institute of Traditional Chinese Veterinary Medicine and MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, PR China. Electronic address: liujiaguo@njau.edu.cn.

PMID: 30658794
DOI: 10.1016/j.carbpol.2018.12.035

PI3KC3-dependent autophagosomes formation pathway is of crucial importance to anti-DHAV activity of Chrysanthemum indicum polysaccharide

Ke Ming et al. Carbohydr Polym. 2019.

. 2019 Mar 15:208:22-31.

doi: 10.1016/j.carbpol.2018.12.035. Epub 2018 Dec 13.

Authors

Ke Ming¹, Wenjuan Yuan², Yun Chen³, Hongxu Du⁴, Miao He⁵, Yuanliang Hu⁶, Deyun Wang⁷, Yi Wu⁸, Jiaguo Liu⁹

Affiliations

¹ Institute of Traditional Chinese Veterinary Medicine and MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, PR China. Electronic address: 2017207037@njau.edu.cn.
² Institute of Traditional Chinese Veterinary Medicine and MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, PR China. Electronic address: 2016107097@njau.edu.cn.
³ College of Animal Science and Technology, College of Tropical Agriculture and Forestry, Hainan University, Hainan Key Lab of Tropical Animal Reproduction & Breeding and Epidemic Disease Research, Animal Genetic Engineering Key Lab of Haikou, Haikou, 570228, PR China. Electronic address: ychen@hainu.edu.cn.
⁴ Institute of Traditional Chinese Veterinary Medicine and MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, PR China. Electronic address: 2016207035@njau.edu.cn.
⁵ Institute of Traditional Chinese Veterinary Medicine and MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, PR China. Electronic address: 2017107099@njau.edu.cn.
⁶ Institute of Traditional Chinese Veterinary Medicine and MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, PR China. Electronic address: ylhu@njau.edu.cn.
⁷ Institute of Traditional Chinese Veterinary Medicine and MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, PR China. Electronic address: dywang@njau.edu.cn.
⁸ Institute of Traditional Chinese Veterinary Medicine and MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, PR China. Electronic address: wuyi2001cn@163.com.
⁹ Institute of Traditional Chinese Veterinary Medicine and MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, PR China. Electronic address: liujiaguo@njau.edu.cn.

PMID: 30658794
DOI: 10.1016/j.carbpol.2018.12.035

Abstract

We previously reported that Chrysanthemum indicum polysaccharide (CIPS) effectively inhibited the replication of duck hepatitis A virus (DHAV). However, the inhibition mechanisms are still unclear. Autophagy plays important role in virus genomic replication. Therefore, in present study, the effect of autophagy on DHAV genome replication as well as the influence of CIPS on autophagy were studied. qPCR, western blot, and ELISA methods were applied to observe the autophagy and analyze the inhibition mechanisms of CIPS on DHAV. Results showed that DHAV infection increased the expression level of LC3-II and interdicted the degradation of p62. Treating with rapamycin benefited DHAV gene expression level. What's more, DHAV infection and rapamycin treatment also promoted the expression of PI3KC3 and increased the concentration of PI3P. However, CIPS treatment significantly downregulated the expressions of LC3-II and PI3KC3 induced by DHAV and rapamycin, and consequently inhibited autophagosomes formation. As a result, DHAV replication was inhibited.

Keywords: Autophagosomes; Chrysanthemum indicum polysaccharide; Duck hepatitis A virus; PI3KC3.

PubMed Disclaimer

Cited by

The DHAV-1 protein VP1 interacts with PI3KC3 to induce autophagy through the PI3KC3 complex.
Li J, Wang M, Zhou S, Cheng A, Ou X, Sun D, Wu Y, Yang Q, Gao Q, Huang J, Tian B, Mao S, Zhang S, Zhao X, Jia R, Liu M, Zhu D, Chen S, Liu Y, Yu Y, Zhang L, Pan L. Li J, et al. Vet Res. 2022 Aug 17;53(1):64. doi: 10.1186/s13567-022-01081-6. Vet Res. 2022. PMID: 35978392 Free PMC article.
Phytochemistry, Pharmacology and Quality Control of Xiasangju: A Traditional Chinese Medicine Formula.
Wu S, Luo H, Zhong Z, Ai Y, Zhao Y, Liang Q, Wang Y. Wu S, et al. Front Pharmacol. 2022 Jun 23;13:930813. doi: 10.3389/fphar.2022.930813. eCollection 2022. Front Pharmacol. 2022. PMID: 35814215 Free PMC article. Review.
The Multi-Faceted Role of Autophagy During Animal Virus Infection.
Jiang H, Kan X, Ding C, Sun Y. Jiang H, et al. Front Cell Infect Microbiol. 2022 Mar 25;12:858953. doi: 10.3389/fcimb.2022.858953. eCollection 2022. Front Cell Infect Microbiol. 2022. PMID: 35402295 Free PMC article. Review.
The inhibitory effect of phosphorylated Codonopsis pilosula polysaccharide on autophagosomes formation contributes to the inhibition of duck hepatitis A virus replication.
Ming K, He M, Su L, Du H, Wang D, Wu Y, Liu J. Ming K, et al. Poult Sci. 2020 Apr;99(4):2146-2156. doi: 10.1016/j.psj.2019.11.060. Epub 2020 Feb 8. Poult Sci. 2020. PMID: 32241500 Free PMC article.
Autophagy in farm animals: current knowledge and future challenges.
Tesseraud S, Avril P, Bonnet M, Bonnieu A, Cassar-Malek I, Chabi B, Dessauge F, Gabillard JC, Perruchot MH, Seiliez I. Tesseraud S, et al. Autophagy. 2021 Aug;17(8):1809-1827. doi: 10.1080/15548627.2020.1798064. Epub 2020 Jul 30. Autophagy. 2021. PMID: 32686564 Free PMC article. Review.

See all "Cited by" articles

MeSH terms

Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions

Substances

Actions
Actions
Actions
Actions
Actions

LinkOut - more resources

Full Text Sources
- Elsevier Science
Research Materials
- Addgene Non-profit plasmid repository
- NCI CPTC Antibody Characterization Program

Save citation to file

Email citation

Add to Collections

Add to My Bibliography

Your saved search

Create a file for external citation management software

Your RSS Feed

PI3KC3-dependent autophagosomes formation pathway is of crucial importance to anti-DHAV activity of Chrysanthemum indicum polysaccharide

Affiliations

PI3KC3-dependent autophagosomes formation pathway is of crucial importance to anti-DHAV activity of Chrysanthemum indicum polysaccharide

Authors

Affiliations

Abstract

Similar articles

Cited by

MeSH terms

Substances

LinkOut - more resources

Full Text Sources

Research Materials

Abstract

Similar articles

Cited by

MeSH terms

Substances

Related information

LinkOut - more resources

Full Text Sources

Research Materials