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. 2019 Jan 1;10(1):43-50.
doi: 10.7150/jca.26723. eCollection 2019.

Circulating miR-1290 and miR-320d as Novel Diagnostic Biomarkers of Human Colorectal Cancer

Xiangxiang Liu  1 , Xueni Xu  2 , Bei Pan  1 , Bangshun He  1 , Xiaoxiang Chen  2 , Kaixuan Zeng  2 , Mu Xu  1 , Yuqin Pan  1 , Huiling Sun  1 , Tao Xu  1 , Xiuxiu Hu  2 , Shukui Wang  1   2
Affiliations

Circulating miR-1290 and miR-320d as Novel Diagnostic Biomarkers of Human Colorectal Cancer

Xiangxiang Liu et al. J Cancer. .

Abstract

Background: The lack of screening methods with high diagnostic utility leads to colorectal cancer (CRC) patients usually diagnosed in advanced stages which results in high mortality. This study aimed to identify novel circulating miRNAs as biomarkers for the early detection of CRC. Materials and Methods: Total 205 participants were enrolled in this study. First, two dysregulated candidate miRNAs were selected after integrated analysis of four GEO datasets. Then, the expression of these two miRNAs in plasma samples were tested through qRT-PCR. Training phase and validation phase were designed to verify the diagnostic value of these two miRNAs using receiver operating characteristic curve (ROC) analysis. Results: After integrated analysis of GEO datasets, we discovered miR-1290 and miR-320d were dysregulated in colorectal adenoma and adenocarcinoma tissues, and circulating miR-1290 and miR-320d in CRC patients were tumor-derived. Thereafter, circulating miR-1290 and miR-320d were selected to further investigate their potential for early diagnosis of CRC. Plasma miR-1290 expression could differentiate adenoma and CRC patients from healthy controls with area under the curve (AUC) of 0.78 and 0.88. Similarly, plasma miR-320d expression could discriminate adenoma and CRC patients from healthy controls with AUC of 0.74 and 0.81. Conclusions: Circulating miR-1290 and miR-320d are novel promising biomarkers for early diagnosis of CRC.

Keywords: circulation; colorectal cancer; diagnostic biomarker; microRNA.

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Conflict of interest statement

Competing Interests: The authors have declared that no competing interest exists.

Figures

Figure 1
Figure 1
The flow diagram of this study designed.
Figure 2
Figure 2
Dysregulated miR-1290 and miR-320d were selected to further explore their potential diagnostic value after integrated analysis of GEO datasets. A-B. Partial miRNAs which were significantly dysregulated in CRC tissues. C. Circulating miRNAs that differentially expressed after operation. D-E. Dysregulated miRNAs in both adenoma and CRC tissues. F. miR-1290 and miR-320d were significantly dysregulated in both adenoma and CRC tissues. *P<0.05, **P<0.01, ***P<0.001.
Figure 3
Figure 3
Exploration to identify circulating miR-1290 and miR-320d as novel biomarkers for early diagnosis of CRC. A-B. Plasma miR-1290 and miR-320d were significantly dysregulated in both adenoma and CRC patients. C-D. The diagnostic utility of plasma miR-1290 and miR-320d to distinguish adenoma and CRC patients from healthy controls. ***P<0.001.
Figure 4
Figure 4
Further validation of tumor-derived circulating miR-1290 and miR-320d as novel biomarkers for early diagnosis of CRC. A-B. Tissue miR-1290 and miR-320d were significantly dysregulated in both adenoma and CRC patients. C-D. The expression of miR-1290 and miR-320d returned to normal levels after surgical resection of malignancies. E-F. The positive correlation of miR-1290 and miR-320d expression levels between matched primary CRC tissues and plasma samples. G-H. Plasma miR-1290 and miR-320d were significantly dysregulated in both adenoma and CRC patients. I-J. The diagnostic utility of plasma miR-1290 and miR-320d to distinguish adenoma and CRC patients from healthy controls. *P<0.05, **P<0.01, ***P<0.001.
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