Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2019 Jan 11:12:221-227.
doi: 10.2147/IDR.S188527. eCollection 2019.

Molecular characterization of the pilS2 gene and its association with the frequency of Pseudomonas aeruginosa plasmid pKLC102 and PAPI-1 pathogenicity island

Aghil Bahramian  1   2 , Saeed Khoshnood  3   4 , Aref Shariati  5 , Farahnoosh Doustdar  2 , Alireza Salimi Chirani  2 , Mohsen Heidary  5   6
Affiliations

Molecular characterization of the pilS2 gene and its association with the frequency of Pseudomonas aeruginosa plasmid pKLC102 and PAPI-1 pathogenicity island

Aghil Bahramian et al. Infect Drug Resist. .

Abstract

Introduction: Pseudomonas aeruginosa is the most common opportunistic pathogen associated with a broad range of infections, including cystic fibrosis, ocular, otitis media, and burn infections. The aim of this study was to show the frequency of the pilS2 gene, and its association with P. aeruginosa plasmid pKLC102 and PAPI-1 pathogenicity island among P. aeruginosa strains.

Methods: The samples were collected from patients with cystic fibrosis, ocular, otitis media, and burn infections between January 2016 and November 2017. DNA was extracted using the DNA extraction kit and was used for PCR assay. PCR with 4 primer-pairs including 976 F/PAPI-1R, 4542 F/intF, SojR/4541 F, and intF/sojR was performed to identify PAPI-1. pKLC102 was detected using three other primer-pairs including cp10F/cp10R, cp44F/cp44R, and cp97F/cp97R.

Results: A total of 112 P. aeruginosa isolates were collected from patients with cystic fibrosis (36), burn (20), otitis media (26), and ocular (30) infections. The results of PCR showed that pilS2 gene was identified in 96 (85%) strains. PAPI-1-attB integration was detected among 38 (33.9%) isolates and the circular form of PAPI-1 detected among 17 (14%) isolates. In addition, 79 (70.5%) strains were found to be positive for pKLC102.

Conclusion: We found that the majority of the isolates may be susceptible to transfer this significant island and the related element pKLC102 into recipient isolates lacking the island owing to high association of the PilS2 pilus with the islands in the studied strains. It is anticipated that strains isolated from burn and eye with the highest rate of PilS2, PAPI-1, and pKLC102 association have a high level of antibiotic resistance.

Keywords: PAPI-1; Pseudomonas aeruginosa; cystic fibrosis; pKLC102; pilS2.

PubMed Disclaimer

Conflict of interest statement

Disclosure The authors report no conflicts of interest in this work.

Figures

Figure 1
Figure 1
Detection of pils2 gene (428 bp) in Pseudomonas aeruginosa isolates. Notes: 1–3 and 5 positive samples, 4 and 6 negative samples; molecular pattern: 100 bp. Abbreviations: N, negative control; P, positive control.
Figure 2
Figure 2
Detection of CP44 gene (272 bp) in Pseudomonas aeruginosa isolates. Note: Molecular pattern: 100 bp. Abbreviations: N, negative control; P, positive control.
Figure 3
Figure 3
Detection of CP10 gene (255 bp) in Pseudomonas aeruginosa isolates. Note: Molecular pattern: 100 bp. Abbreviations: N, negative control; P, positive control.
See this image and copyright information in PMC

References

    1. Heidary M, Hashemi A, Goudarzi H, et al. The antibacterial activity of Iranian plants extracts against metallo beta-lactamase producing Pseudomonas aeruginosa strains. J Paramed Sci. 2016;7(1):13–19.
    1. Wang MC, Liu CY, Shiao AS, Wang T. Ear problems in swimmers. J Chin Med Assoc. 2005;68(8):347–352. - PubMed
    1. Buchele MLC, Wopereis DB, Casara F, et al. Contact lens-related polymicrobial keratitis: Acanthamoeba spp. genotype T4 and Candida albicans. Parasitol Res. 2018;117(11):3431–3436. - PubMed
    1. Tarashi S, Heidary M, Dabiri H, Nasiri MJ. Prevalence of drug-resistant Pseudomonas aeruginosa in Iranian burned patients: a meta-analysis. Arch Trauma Res. 2017;6(3):1–17.
    1. Chirani AS, Majidzadeh R, Pouriran R, et al. The effect of in silico targeting Pseudomonas aeruginosa patatin-like protein D, for immunogenic administration. Comput Biol Chem. 2018;74:12–19. - PubMed

LinkOut - more resources