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. 2019 Mar;137(3):527-530.
doi: 10.1007/s00401-018-01955-0. Epub 2019 Jan 21.

Antisense RNA foci are associated with nucleoli and TDP-43 mislocalization in C9orf72-ALS/FTD: a quantitative study

Olubankole Aladesuyi Arogundade  1 , Jennifer E Stauffer  1   2 , Shahram Saberi  1   3 , Sandra Diaz-Garcia  1 , Sahana Malik  1   4 , Hani Basilim  1   5 , Maria J Rodriguez  1 , Takuya Ohkubo  1 , John Ravits  6
Affiliations

Antisense RNA foci are associated with nucleoli and TDP-43 mislocalization in C9orf72-ALS/FTD: a quantitative study

Olubankole Aladesuyi Arogundade et al. Acta Neuropathol. 2019 Mar.
No abstract available

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Conflict of interest statement

Conflict of Interest

The authors declare that they have no conflict of interest.

Figures

Fig. 1
Fig. 1. Antisense RNA foci have unique neuropathological features compared to sense RNA foci.
(a): Sense and antisense RNA foci (arrowheads) are observed in the nucleoplasm or on the periphery of nucleoli of neurons (outer, middle and innermost dashed lines mark the cytoplasm, nucleoplasm and nucleolus, respectively). (b): Nucleolar localization is confirmed by nucleolar marker nucleolin. (c and d): Neurons containing antisense RNA foci often have cytoplasmic aggregation of TDP-43 and neurons with sense RNA foci often have preserved nuclear TDP-43 (inset scale, 500 nm). (e): The percentage of neurons with nucleolar antisense RNA foci was significantly increased in neurons with cytoplasmic aggregation of TDP-43 compared to preserved nuclear TDP-43 (p = 0.008). (f): The percentage of neurons with nucleolar sense RNA foci was not significantly different in neurons with cytoplasmic aggregation of TDP-43 compared to preserved nuclear TDP-43 (p = 0.7, Mann­Whitney test). (g): The co-occurrence of nucleolar antisense RNA foci and cytoplasmic aggregation of TDP-43 is significantly greater than expected while the co-occurrence of nucleolar sense RNA foci and cytoplasmic aggregation of TDP-43 is not. Area proportional Venn-diagrams represent Fisher’s exact test. Data is from motor neurons within the anterior horn of spinal cord.
See this image and copyright information in PMC

References

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Supplementary concepts