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. 2019 Jan;83(1):68-74.

Protective efficacy of an inactivated Brucella abortus vaccine candidate lysed by GI24 against brucellosis in Korean black goats

Wong-Kyong Kim  1 , Ja-Young Moon  1 , Jeong-Sang Cho  1 , Enkhsaikhan Ochirkhuyag  1 , Md Rashedunnabi Akanda  1 , Byung-Yong Park  1 , Jin Hur  1
Affiliations

Protective efficacy of an inactivated Brucella abortus vaccine candidate lysed by GI24 against brucellosis in Korean black goats

Wong-Kyong Kim et al. Can J Vet Res. 2019 Jan.

Abstract
in English, French

The efficacy of GI24-lysed Brucella abortus cells as a vaccine candidate against brucellosis in goats was evaluated on 2 groups of Korean black goats. Group A goats were immunized subcutaneously (SC) with sterile phosphate-buffered saline, whereas group B goats were immunized SC with approximately 3 × 109 lysed B. abortus cells. Subcutaneous immunization with the lysed cells did not cause any negative impact on the overall clinical status, such as behavior and appetite, throughout the study period. The enzyme-linked immunosorbent assay (ELISA) optical densities values for B. abortus lipopolysaccharide in serum were considerably higher in group B than those in group A. Also, the levels of the cytokines interleukin 4 (IL-4), tumor necrosis factor-alpha (TNF-α), and interferon gamma (IFN-γ) were significantly elevated in group B compared with those in group A. Following intraconjunctival challenge with B. abortus strain 544, the severity of brucellosis in terms of infection index and colonization of B. abortus in tissues was significantly lower in group B than in group A. The present study concluded that 3 of 5 goats immunized with GI24-lysed bacteria were completely protected against challenge. Future investigations are required to improve the protective efficacy offered by lysed B. abortus cells for practical applications in small ruminants.

L’efficacité de cellules lysées de Brucella abortus GI24 comme vaccin candidat contre la brucellose chez les chèvres a été évaluée chez deux groupes de chèvres noires coréennes. Les chèvres du groupe A ont été immunisées par voie sous-cutanée (SC) avec de la saline tamponnée stérile, alors que les chèvres du groupe B ont été immunisées SC avec environ 3 × 109 cellules lysées de B. abortus. L’immunisation sous-cutanée avec les cellules lysées n’a pas eu d’impact négatif sur l’état clinique général, tel que le comportement et l’appétit, tout au long de la période d’étude. Les valeurs de densité optique obtenues lors d’épreuves immunoenzymatiques (ELISA) utilisant le lipopolysaccharide de B. abortus étaient considérablement plus élevées avec le sérum des animaux du groupe B que celui des animaux du groupe A. De plus, les niveaux des cytokines interleukine-4 (IL-4), du facteur-alpha nécrosant de tumeur (TNF-α), d’interféron-gamma (IFN-γ) étaient significativement plus élevés dans le groupe B comparativement au groupe A. Pour donner suite à l’infection-défi intra-conjonctivale avec la souche 544 de B. abortus, la sévérité de brucellose en termes d’index d’infection et de colonisation des tissus par B. abortus était significativement moindre dans le groupe B que dans le groupe A. La présente étude a permis de conclure que 3 des 5 chèvres immunisées avec les bactéries GI24 lysées étaient complètement protégées contre l’infection. Des études ultérieures sont requises pour améliorer l’efficacité protectrice offerte par les cellules lysées de B. abortus pour une application pratique chez les petits ruminants.(Traduit par Docteur Serge Messier).

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Figures

Figure 1
Figure 1
Transmission electron micrographs of B. abortus biotype 1 treated with GI24. A — The untreated B. abortus cells. B — B. abortus cells treated with GI24. The bacterial cells were incubated with 40 μg/mL of GI24 for 30 h at 37°C.
Figure 2
Figure 2
The optical density (OD) of ELISA against B. abortus LPS. Group A goats were inoculated with 1.0 mL of sterile PBS as a control. Group B goats were immunized subcutaneously with approximately 3 × 109 GI24-lysed B. abortus cells in 1 mL PBS. Data represent the means of all goats in each group and error bars represent the standard deviations (SD). Asterisks indicate a significant difference between the values obtained for the immunized group and those obtained for the control group (*P < 0.05).
Figure 3
Figure 3
IL-4, TNF-α, and IFN-γ concentrations (pg/mL) in peripheral blood mononuclear cells at 6 wk post prime immunization. Group A goats were inoculated with 1.0 mL of sterile PBS as a control. Group B goats were immunized subcutaneously with approximately 3 × 109 GI24-lysed B. abortus cells in 1 mL PBS. Data represent the means of all goats in each group and error bars indicate the standard deviations (SD). Asterisks indicate significant differences among the values obtained for each group (*P < 0.05).
Figure 4
Figure 4
A — Index of infection for goats challenged with B. abortus strain 544 at 6 wk post prime immunization. B — Colonization and incidence of recovery of B. abortus strain 544 in tissues. Group A goats were inoculated with 1.0 mL of sterile PBS as a control. Group B goats were immunized subcutaneously with approximately 3 × 109 GI24-lysed B. abortus cells in 1 mL PBS. All goats in each group were intraconjunctivally challenged with 1 × 107 CFU of virulent wild-type B. abortus 544 at 6 wk post prime immunization. The numbers of viable bacteria recovered from each tissue of goats at 12 wk post challenge are shown (LN — lymph node).
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