Induction of superovulation in mature mice and rats using serum of spayed female dogs

Abstract

The following experiments were designed to examine the effect of serum of spayed dogs on superovulation response in mice and rats. In Experiment 1, female mice at diestrus (n=30) were divided into three equal groups and superovulated with either administration of 5 IU pregnant mare serum gonadotropin (PMSG) or recombinant follicle stimulating hormone (rFSH) (reducing dose from 2.5 to 0.5 IU) and 5 IU human chorionic gonadotropin (hCG) administered 48h later. Serum of spayed dogs was administered intraperitoneally at a reduced dose from 0.1 to 0.025 mL in a 48 h period. In Experiment 2, female rats (n=30) at diestrus stage were divided into three equal groups. Superovulation was induced using either 30 IU PMSG, or a dose reduced from 5 to 1 IU rFSH and 25 IU hCG administered 48h later. Serum of spayed dogs was administered in a reduced dose from 0.6 to 0.1 mL in a 48 hour period. Female mice and rats were mated 24 h following hCG administration. On day 14 after mating, animals were euthanized and ovarian sections were fixed for histopathological evaluation and corpus luteum (CL) counting. No significant difference observed in mean (±SEM) number of CLs between the PMSG group and the mice that received serum of spayed dog (10.4±1.3 vs 9.2±1.0). Mean (±SEM) number of CLs tended to be lower in rats that received serum of spayed dog than those of rats which received either PMSG or rFSH (15.1±1.9 vs 23.6±3.1 and 23.1±2.9, P=0.06, respectively). In conclusion, serum of spayed dogs is able to induce a superovulatory response in mice and rats.

Keywords: FSH; PMSG; Superovulation; spayed dog serum.

Figure 1. Experimental design indicated that mice (n=30) and rats (n=30) aligned into three equal groups and received PMSG, rFSH or spayed dog serum and hCG.

Figure 2. Individual variation of FSH concentration (Mean±SEM) during 6 months following female dog spaying. The pattern of variations was similar in two dogs, but FSH concentration was different.

Figure 3. Mean number of CLs in different groups of mice following superovulation with FSH, PMSG and serum of spayed dogs. Significant difference was observed between rFSH group and two other groups (P>0.05).

Figure 4. Mean number of CLs in different groups of rats following superovulation with FSH, PMSG and serum of spayed dogs. There was no significant difference among treated groups.