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. 2018 Dec;34(4):216-222.
doi: 10.5625/lar.2018.34.4.216. Epub 2018 Dec 31.

In vitro and in vivo inhibition of Helicobacter pylori by Lactobacilllus paracasei HP7

Seong-Soo Hong  1 , Hyun-A Lee  2 , Joo Yun Kim  3 , Ji-Woong Jeong  3 , Jae-Jung Shim  3 , Jung Lyoul Lee  3 , Jae-Hun Sim  3 , Yungho Chung  4 , Okjin Kim  2
Affiliations

In vitro and in vivo inhibition of Helicobacter pylori by Lactobacilllus paracasei HP7

Seong-Soo Hong et al. Lab Anim Res. 2018 Dec.

Abstract

The efficacy of standard therapeutic strategies for Helicobacter pylori (H. pylori) infection is decreasing over time due to the emergence of drug-resistant strains. As an alternative, the present study investigated the capacity of Lactobacilllus paracasei (L. paracasei) HP7, isolated from kimchi, to inhibit H. pylori growth. The effects of L. paracasei HP7 on H. pylori adhesion and H. pylori-induced inflammation were examined in AGS human gastric adenocarcinoma epithelial cells and a mouse model of H. pylori SS1 infection. L. paracasei HP7 reduced H. pylori adhesion to AGS cells and suppressed the inflammatory response in infected cells by downregulating interleukin-8. H. pylori colonization in the stomach of C57BL/6 mice was demonstrated by rapid urease test, and results showed significant decrease in mice post-treated with L. paracasei HP7. Additionally, L. paracasei HP7 decreased gastric inflammation and epithelial lesions in the stomach of H. pylori-infected mice. These results demonstrate that L. paracasei HP7 treatment can inhibit H. pylori growth and is thus a promising treatment for patients with gastric symptoms such as gastritis that are caused by H. pylori infection.

Keywords: AGS cells; HP7; Helicobacter pylori; Kimchi; Lactobacilllus paracasei.

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Conflict of interest statement

Conflict of interests: The authors declare that there is no financial conflict of interests to publish these results.

Figures

Figure 1
Figure 1. Inhibition of H. pylori adhesion to AGS cells by L. paracasei HP7 (HP7). AGS cells pre-treated with L. paracasei HP7 showed lower expression of H. pylori 16S RNA.
Figure 2
Figure 2. Inhibitory effect of L. paracasei HP7 (HP7) on H. pylori-induced IL-8 production. L. paracasei HP7 was added to a confluent layer of AGS cells in a 96-well plate 30 min before adding H. pylori. After incubation for 24 h, the culture supernatant was collected to measure the amount of released IL-8.
Figure 3
Figure 3. Suppression of H. pylori infection in C57BL/6 mice by treatment with L. paracasei HP7. Serum samples collected after sacrifice were evaluated for H. pylori IgG-1 by ELISA. H. pylori IgG-1 levels were decreased in the H. pylori/L. paracasei HP7 group as compared to the H. pylori infection group.
Figure 4
Figure 4. CLO scores for gastric mucosa of mice infected with H. pylori followed by treatment with L. paracasei HP7.
See this image and copyright information in PMC

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