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. 2019 Jan 4:21:e00303.
doi: 10.1016/j.btre.2019.e00303. eCollection 2019 Mar.

16S rRNA-Based metagenomic analysis of microbial communities associated with wild Labroides dimidiatus from Karah Island, Terengganu, Malaysia

Ashyikin Noor Ahmad Nurul  1 , Danish-Daniel Muhammad  1   2 , Victor Tosin Okomoda  3 , Ariffin Asma Bt Nur  1   4
Affiliations

16S rRNA-Based metagenomic analysis of microbial communities associated with wild Labroides dimidiatus from Karah Island, Terengganu, Malaysia

Ashyikin Noor Ahmad Nurul et al. Biotechnol Rep (Amst). .

Erratum in

Abstract

This study was designed to evaluate the bacterial composition of the Labroides dimidiatus and its surrounding water. Fish and carriage water samples were obtained from corals of the Karah Island in Terengganu Malaysia. DNA was extracted and the bacteria communities on the skin mucus and stomach as well as water sample were classified (to family level) using the 16S rRNA-based metagenomics analysis. 1,426,740 amplicon sequence reads corresponding to 508 total operational taxonomic units were obtained from the three metagenomics libraries in this study. The Proteobacteria, Bacteroidetes, Firmicutes, Actinobacteria and Fusobacteria were the most dominant bacterial phyla in all samples. A total of 36 different classes and 132 families were identified, many of which had shared presence in all samples while others were exclusive to different sample. Thirty-three of these were identified as pathogenic zoonotic bacterial. The results obtained indicate a strong influence of host environment on the composition of its microbiota. Knowing the composition of the microbiota is the first step toward exploring proper management of this ornamental fish in captivity.

Keywords: Bacterial community; Bluestreak cleaner wrasse; Microbiota; Skin mucus.

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Figures

Fig. 1
Fig. 1
Taxonomic diversity and relative abundance the different classes of Bacteria associated with Labroides dimiditus from Karah Island, Terengganu Malaysia. PK = Karah Island, M = skin mucus, S = stomach W = carriage water. General = PK-M + PK-S + PK-W (i.e. the cumulative Bacteria classes associated with the Labroides dimiditus as observed in the mucus, stomach and carriage water).
Fig. 2
Fig. 2
Taxonomic diversity and relative abundance at the order level of bacteria associated with skin mucus sample of Labroides dimiditus from Karah Island, Terengganu Malaysia. Each bar denotes individually orders in Alpha-, Beta-, Delta-, Epsilon-, and Gamma-proteobacteria respectively, while the last bar (others) shows orders in others phylum.
Fig. 3
Fig. 3
Taxonomic diversity and relative abundance at the order level of bacteria associated with stomach sample of Labroides dimiditus from Karah Island, Terengganu Malaysia. Each bar denotes individually orders in Alpha-, Beta-, Delta-, Epsilon-, and Gamma-proteobacteria respectively, while the last bar (others) shows orders in others phylum.
Fig. 4
Fig. 4
Taxonomic composition and relative abundance at the order level of bacteria associated with carriage water from Karah Island, Terengganu Malaysia. Each bar denotes individually orders in Alpha-, Beta-, Delta-, Epsilon-, and Gamma-proteobacteria respectively, while the last bar (others) shows orders in others phylum.
Fig. 5
Fig. 5
Venn diagram of the percentages of shared and unique bacteria families identified in three different types of samples collected in this study. PK = Karah Island, M = skin mucus, S = stomach, W = carriage seawater.
See this image and copyright information in PMC

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