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. 2019 Mar;64(1):31-43.
doi: 10.2478/s11686-018-00005-9. Epub 2019 Jan 22.

Immunological Parameters of the Pomacea lineata Spix, 1827 (Mollusca: Caenogastropoda) Exposed to Schistosoma mansoni Sambon, 1907

Bárbara Brooklyn Timóteo Nascimento Silva  1 , Luiz Carlos Alves  2 , Fábio André Brayner  2 , Nairomberg Cavalcanti Portela Junior  2 , Fernanda das Chagas Angelo Mendes Tenório  3 , Aline Cristina Silva Lira  4 , Yuri Mateus Lima de Albuquerque  5 , Clovis José Cavalcanti Lapa Neto  5 , Valéria Wanderley-Teixeira  5 , Álvaro Aguiar Coelho Teixeira  5
Affiliations

Immunological Parameters of the Pomacea lineata Spix, 1827 (Mollusca: Caenogastropoda) Exposed to Schistosoma mansoni Sambon, 1907

Bárbara Brooklyn Timóteo Nascimento Silva et al. Acta Parasitol. 2019 Mar.

Abstract

Introduction: Pomacea lineata acts as the natural biological controller of Biomphalaria glabrata, the intermediate host of Schistosoma mansoni, as they are found in the same environment. However, there are no studies reporting an infection in P. lineata due to S. mansoni. Thus, this work investigated parameters related to the immunity of P. lineata after exposure for 24 and 48 h to S. mansoni under experimental conditions.

Methods: The F1 generation of these snails was used in this study. The total and differential counts of hemocytes, phenoloxidase, nitric oxide, total proteins, expression of TNF-α in hemocytes and histopathology of the head-foot organ were analyzed.

Results: Exposure to S. mansoni promoted an increase in the total number of hemocytes, an increase of granulocytes, a reduction of agranulocytes and hyalinocytes, an increase in phenoloxidase levels, total proteins and nitric oxide. There was TNF-α expression in the agranulocytes and granulocytes, increasing in intensity after exposure to the trematode. Head-foot histopathology revealed the presence of sporocytes in the fibromuscular layer surrounded by granulation tissue only within 24 h. At 48 h, there was marked fibrosis in this layer and little granulation tissue.

Conclusion: Thus, we can conclude that P. lineata seems to trigger a series of immunological strategies in a very effective way that confers some resistance to S. mansoni.

Keywords: Hemocytes; Miracidia; Pomacea lineata; Resistance; Schistosoma.

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