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. 2019 Jan 23;14(1):e0211002.
doi: 10.1371/journal.pone.0211002. eCollection 2019.

Genome constellations of 24 porcine rotavirus group A strains circulating on commercial Thai swine farms between 2011 and 2016

Supansa Tuanthap  1 , Sompong Vongpunsawad  2 , Supol Luengyosluechakul  3 , Phanlert Sakkaew  4 , Apiradee Theamboonlers  2 , Alongkorn Amonsin  5 , Yong Poovorawan  2
Affiliations

Genome constellations of 24 porcine rotavirus group A strains circulating on commercial Thai swine farms between 2011 and 2016

Supansa Tuanthap et al. PLoS One. .

Abstract

Rotavirus A (RVA) infection is a major cause of diarrhea-related illness in young children. RVA is also one of the most common enteric viruses detected on pig farms and contributes to substantial morbidity and mortality in piglets. Long-term multi-site surveillance of RVA on Thai swine farms to determine the diversity of RVA strains in circulation is currently lacking. In this study, we characterized the 11 segments of the RVA genome from 24 Thai porcine RVA strains circulating between 2011 and 2016. We identified G9 (15/24) and P[13] (12/24) as the dominant genotypes. The dominant G and P combinations were G9P[13] (n = 6), G9P[23] (n = 6), G3P[13] (n = 5), G9P[19] (n = 3), G4P[6] (n = 2), G4P[19] (n = 1), and G5P[13] (n = 1). Genome constellation of the Thai strains showed the predominance of Wa-like genotype (Gx-P[x]-I1/I5-R1-C1-M1-A8-N1-T1/T7-E1/E9-H1) with evidence of reassortment between the porcine and human RVA strains (e.g., G4-P[6]-I1-R1-C1-M1-A8-N1-T1-E1-H1 and G9-P[19]-I5-R1-C1-M1-A8-N1-T7-E9-H1). To assess the potential effectiveness of rotavirus vaccination, the Thai RVA strains were compared to the RVA strains represented in the swine rotavirus vaccine, which showed residue variations in the antigenic epitope on VP7 and shared amino acid identity below 90% for G4 and G5 strain. Several previous studies suggested these variations might effect on virus neutralization specificity and vaccine efficacy. Our study illustrates the importance of RVA surveillance beyond the G/P genotyping on commercial swine farms, which is crucial for controlling viral transmission.

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Conflict of interest statement

The authors have declared that no competing interests exist.

Figures

Fig 1
Fig 1. Phylogenetic analysis of the RVA VP7 gene.
The nucleotide sequences of the Thai strains (black dotted) were compared to those of previous Thai porcine RVA strains (red dotted), the RVA reference and vaccine strains (blue).
Fig 2
Fig 2. Phylogenetic analysis of the RVA VP4 gene.
The nucleotide sequences of the Thai strains (dotted) were compared to those of previous Thai porcine RVA strains (red dotted), the RVA reference and vaccine strains (blue).
Fig 3
Fig 3. Phylogenetic analysis of the RVA VP6 gene.
The nucleotide sequences of the Thai strains (dotted) were compared to those of previous Thai porcine RVA strains (red dotted), the RVA reference and vaccine strains (blue).
Fig 4
Fig 4. Phylogenetic analysis of the RVA VP1, VP2, and VP3 genes.
The nucleotide sequences of the Thai strains (dotted) were compared to those of previous Thai porcine RVA strains (red dotted), the RVA reference and vaccine strains (blue).
Fig 5
Fig 5. Phylogenetic analysis of the RVA non-structural genes.
The nucleotide sequences of the Thai strains (dotted) were compared to those of previous Thai porcine RVA strains (red dotted), the RVA reference and vaccine strains (blue).
Fig 6
Fig 6. Residue differences in the VP7 antigenic regions between the Thai strains and the RVA reference/vaccine strains.
Residue positions for each region are numbered. Identical residues are indicated by dots.
See this image and copyright information in PMC

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References

    1. Collaborators GBDDD. Estimates of global, regional, and national morbidity, mortality, and aetiologies of diarrhoeal diseases: a systematic analysis for the Global Burden of Disease Study 2015. Lancet Infect Dis. 2017;17(9):909–48. Epub 2017/06/06. 10.1016/S1473-3099(17)30276-1 - DOI - PMC - PubMed
    1. Vlasova AN, Amimo JO, Saif LJ. Porcine Rotaviruses: Epidemiology, Immune Responses and Control Strategies. Viruses. 2017;9(3). Epub 2017/03/25. 10.3390/v9030048 - DOI - PMC - PubMed
    1. Pesavento JB, Crawford SE, Estes MK, Prasad BV. Rotavirus proteins: structure and assembly. Curr Top Microbiol Immunol. 2006;309:189–219. Epub 2006/08/18. . - PubMed
    1. Estes M.K and Greenberg HB. Rotaviruses In: Knipe DM, Howley P.M., et al. (Eds.), editor. Fields virology. sixth ed Philadelphia: Wolters Kluwer—Lippincott Williams & Wilkins; 2013. p. 1347–401.
    1. Matthijnssens J, Otto PH, Ciarlet M, Desselberger U, Van Ranst M, Johne R. VP6-sequence-based cutoff values as a criterion for rotavirus species demarcation. Arch Virol. 2012;157(6):1177–82. Epub 2012/03/21. 10.1007/s00705-012-1273-3 . - DOI - PubMed

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