A freeze-and-thaw method to reuse agarose gels for DNA electrophoresis

Abstract

A novel protocol to reuse agarose following agarose gel electrophoresis was established in this study. By repeated freeze-and-thaw of the agarose gel, ethidium bromide and other buffer components in the gel were safely removed from the gel without generation of any toxic fume. The agarose recovered using this method can be used for further electrophoretic experiments without any issues.

Keywords: Agarose; agarose gel electrophoresis; ethidium bromide.