Antidiarrheal Efficacy of Probiotic Bacteria in Castor Oil Induced Diarrheal Mice

Abstract

Probiotics may offer a safe intervention for diarrheal diseases. The aim of the present study was the assessment of the antidiarrheal property of probiotic bacteria. For their antidiarrheal efficacy assessment, yogurt was prepared using the isolated bacteria from selective regional yogurt of Bangladesh, and mice model trails were conducted using castor oil induced diarrheal mice. The probiotic treatment was applied on three mice groups, each having 6 mice and their respective doses were 50 mL/kg body weight in treatment group (TG) 1, 100 mL/kg body weight in TG2, and 150 mL/kg body weight in TG3. A four week treatment of probiotic significantly (P<0.001) reduced the percentage (67.37%) of diarrhea in TG3 (150 mL yogurt/kg body weight). All the treatment groups showed a significant (P<0.001) increase in the latent periods, reduced the total fecal output, and frequency and fecal water content compared to the negative control group. Serum electrolytes (Na⁺ and K⁺) and total protein levels were higher in the TG3 compared to the negative control group. Further research regarding molecular characterization and identification of specific genes and proteins of interest may help to develop the next generation bacteriocins and antidiarrheal drugs.

Keywords: diarrhea; electrolytes; probiotics; treatment; yogurt.

Fig. 1

Mean fecal output (g) from 1st to 4th hour after induction of castor oil. The feces were collected from the blotting paper lined under the cage after administration of castor oil orally and weighted in order to measure the total amount of defecation in the mice groups. Values are mean±SEM. NC, negative control; PC, positive control; Std., standard; TG1, treatment group with 50 mL/kg of yogurt; TG2, treatment group with 100 mL/kg of yogurt; TG3, treatment group with 150 mL/kg of yogurt.