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. 2018 Dec;23(4):335-340.
doi: 10.3746/pnf.2018.23.4.335. Epub 2018 Dec 31.

Chemical Composition and Antioxidant Activity of Crude Polysaccharide from Citron (Citrus junos Sieb. Ex TANAKA) Seed

Seong Yeong Kim  1
Affiliations

Chemical Composition and Antioxidant Activity of Crude Polysaccharide from Citron (Citrus junos Sieb. Ex TANAKA) Seed

Seong Yeong Kim. Prev Nutr Food Sci. 2018 Dec.

Abstract

The crude polysaccharide (citron seed crude polysaccharide, CSCP) from citron (Citrus junos Sieb. Ex TANAKA) seed was obtained by water extraction and ethanol precipitation. The results of chemical composition analyses of the CSCP observed that neutral sugar content and uronic acid content were 27.70% and 14.70%, respectively. Monosaccharide composition of the CSCP was as follows in the following order: galacturonic acid+glucuronic acid (14.70 mol%)> arabinose (12.85 mol%)> glucose (4.54 mol%)> galactose (4.32 mol%)> mannose (2.70 mol%)> fucose (1.68 mol%)> rhamnose (1.61 mol%). The total polyphenol contents of the CSCP was 30.42 μg/mL and 86.12 μg/mL at 1 mg/mL and 10 mg/mL, respectively (P<0.001). 2,2'-Azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activity was higher at the concentration of the CSCP 10 mg/mL (47.12%) than that of the CSCP 1 mg/mL (14.02%), (P<0.001). The ABTS radical scavenging activity was higher than 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity (14.33%) at the same concentration of the CSCP 10 mg/mL (P<0.001). Consequently, in this study, the crude polysaccharide from citron seed may be as a novel potential antioxidant.

Keywords: 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid); antioxidant activity; citron seed; crude polysaccharide; monosaccharide composition.

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Conflict of interest statement

AUTHOR DISCLOSURE STATEMENT The author declares no conflict of interest.

Figures

Fig. 1
Fig. 1
Gas chromatography chromatogram of the standards (A) and the citron seed crude polysaccharide (B) for analysis of monosaccharide composition.
Fig. 2
Fig. 2
Total polyphenol content of the citron seed crude polysaccharide. ***Significantly different at P <0.001 by t -test.
Fig. 3
Fig. 3
Antioxidant activities of the citron seed crude polysaccharide. ABTS; 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging activity, DPPH; 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity. Different letters (a,b) mean significant differences among samples at P <0.001 by ANOVA test.
See this image and copyright information in PMC

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