Preparation and properties of carbon nanotube (Fe)/hydroxyapatite composite as magnetic targeted drug delivery carrier

Abstract

A novel magnetic targeted drug delivery carrier based on a carbon nanotube (Fe)/hydroxyapatite (CNT(Fe)/HA) composite was successfully fabricated by an in situ synthesis of CNTs in HA nanopowder using Fe catalysts and subsequent chemical modification of the as-fabricated CNT(Fe)/HA by chitosan (CS) and folic acid (FA) for a controllable release of an anticancer drug doxorubicin (DOX). The synthesized CNTs, Fe, and HA self-assembled into a composite structure in situ during the synthesis. After the acid treatment, the CNTs were shorter and homogeneously dispersed, the tips of the CNTs were opened, and oxygen-containing functional groups were introduced onto the CNTs. Upon the functional modification through the surface coating with CS and FA, the functionalized CNT(Fe)/HA became capable of loading DOX through both π-π stacking and electrostatic adsorption of FA. The results showed that the average drug-loading rate of DOX was 130 wt%. Furthermore, the pH response of FA-CS-CNT(Fe)/HA enabled the release of a large amount of DOX in phosphate-buffered saline (PBS) at pH = 5.5 with an average drug release rate of 52 wt% after 72 h. In contrast, the drug release in PBS at pH = 7.4 was only 8 wt%. In addition, the saturation magnetization, coercive force, and remanence to saturation magnetization ratio of DOX-FA-CS-CNT(Fe)/HA were 0.88 emu g^-1, 668.96 Oe, and 0.44, respectively, indicating its potential for drug transport under strong external magnetic fields, which could enable magnetic targeted delivery.

Keywords: Carbon nanotube (Fe)/hydroxyapatite composite; Controlled release; Drug delivery carrier; Magnetic property.