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. 2019 Feb;17(2):1390-1394.
doi: 10.3892/etm.2018.7072. Epub 2018 Dec 7.

Value of combined detection of anti-nuclear antibody, anti-double-stranded DNA antibody and C3, C4 complements in the clinical diagnosis of systemic lupus erythematosus

Changhua Qu  1 , Juan Zhang  2 , Xiumei Zhang  3 , Jiexin Du  4 , Baifang Su  5 , Hong Li  6
Affiliations

Value of combined detection of anti-nuclear antibody, anti-double-stranded DNA antibody and C3, C4 complements in the clinical diagnosis of systemic lupus erythematosus

Changhua Qu et al. Exp Ther Med. 2019 Feb.

Abstract

Combined detection of antinuclear antibody (ANA), anti-double-stranded DNA (ds-DNA) antibody and complements C3 and C4 in the diagnosis of systemic lupus erythematosus (SLE) was analyzed. One hundred and ninety-four patients with SLE admitted to Yantaishan Hospital of Yantai from January 2012 to December 2017 were selected as SLE group. A total of 106 patients with non-SLE rheumatic disease were selected as disease control group and 120 healthy subjects as healthy control group. The ANA and anti-ds-DNA antibodies were detected by ELISA and complement C3 and C4 were detected by rate nephelometry. The sensitivity and specificity of these four factors were also analyzed for the diagnosis of SLE. The sensitivity and specificity of ANA in diagnosing SLE were 91.75 and 79.65%, respectively; of anti-ds-DNA antibody were 67.01 and 98.23%, respectively; of complement C3 were 87.11 and 82.74%, respectively; and of complement C4 were 88.66 and 77.43%, respectively. The sensitivity and specificity of ANA and anti-ds-DNA antibody in the diagnosis of SLE were 95.36 and 96.90%, respectively; of C3 and C4 were 92.78 and 79.20%, respectively; and the sensitivity and specificity of the combination of all four indicators were 97.42 and 80.97%, respectively. The combined diagnosis of SLE with ANA, anti-ds-DNA antibody, complement C3 and C4 can play a complementary role in the diagnosis and treatment of SLE patients, and it is of great significance to the diagnosis and treatment planning of SLE patients.

Keywords: anti-double-stranded DNA antibody; antinuclear antibody; complement C3; complement C4; systemic lupus erythematosus.

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Figures

Figure 1.
Figure 1.
ROC curve analysis of the diagnosis of SLE by complement C3 and C4.
Figure 2.
Figure 2.
Diagnostic value of the combined use of complement C3 and C4 in SLE. The ROC curve analysis of the diagnosis of SLE by combination of C3 and C4 is shown.
See this image and copyright information in PMC

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