Making Solid-Phase Peptide Synthesis Greener: A Review of the Literature

Abstract

To date, the synthesis of peptides is concurrent with the production of enormous amounts of toxic waste. DMF, CH₂ Cl₂ , and NMP are three of the most toxic organic solvents used in chemical synthesis and are the most common solvents used for peptide synthesis. Additionally, concerns about the hepatotoxicity caused by exposure to DMF and from the toxic and allergenic nature of additives used in peptide synthesis necessitates the need for a green, environmentally friendly, and safer protocol for peptide synthesis. This review summarizes the current literature on green solid-phase peptide synthesis successes and challenges encountered. The review concludes with suggestions for future research towards a simple and efficient green peptide synthesis protocol.

Keywords: Leuchs’ anhydride; ball-milling; enzymatic synthesis; green solvent; peptide synthesis.