Rapid detection of Lactobacillus crispatus and Lactobacillus iners in vaginal specimens by loop-mediated isothermal amplification

Satoshi Higashide¹, Otomi Cho¹, Yuko Matsuda², Daiki Ogishima³, Sanae Kurakado¹, Takashi Sugita⁴

Affiliations

¹ Department of Microbiology, Meiji Pharmaceutical University, 2-522-1, Noshio, Kiyose, Tokyo 204-8588, Japan.
² Department of Microbiology, Meiji Pharmaceutical University, 2-522-1, Noshio, Kiyose, Tokyo 204-8588, Japan; Department of Obstetrics and Gynecology, Juntendo University Nerima Hospital, 3-1-10 Takanodai, Nerima-ku, Tokyo 177-8521, Japan; Department of Obstetrics and Gynecology, Juntendo University Faculty of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo 113-8421, Japan.
³ Department of Obstetrics and Gynecology, Juntendo University Nerima Hospital, 3-1-10 Takanodai, Nerima-ku, Tokyo 177-8521, Japan; Department of Obstetrics and Gynecology, Juntendo University Faculty of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo 113-8421, Japan.
⁴ Department of Microbiology, Meiji Pharmaceutical University, 2-522-1, Noshio, Kiyose, Tokyo 204-8588, Japan. Electronic address: sugita@my-pharm.ac.jp.

PMID: 30682384
DOI: 10.1016/j.mimet.2019.01.011

Rapid detection of Lactobacillus crispatus and Lactobacillus iners in vaginal specimens by loop-mediated isothermal amplification

Satoshi Higashide et al. J Microbiol Methods. 2019 Mar.

. 2019 Mar:158:18-20.

doi: 10.1016/j.mimet.2019.01.011. Epub 2019 Jan 22.

Authors

Satoshi Higashide¹, Otomi Cho¹, Yuko Matsuda², Daiki Ogishima³, Sanae Kurakado¹, Takashi Sugita⁴

Affiliations

¹ Department of Microbiology, Meiji Pharmaceutical University, 2-522-1, Noshio, Kiyose, Tokyo 204-8588, Japan.
² Department of Microbiology, Meiji Pharmaceutical University, 2-522-1, Noshio, Kiyose, Tokyo 204-8588, Japan; Department of Obstetrics and Gynecology, Juntendo University Nerima Hospital, 3-1-10 Takanodai, Nerima-ku, Tokyo 177-8521, Japan; Department of Obstetrics and Gynecology, Juntendo University Faculty of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo 113-8421, Japan.
³ Department of Obstetrics and Gynecology, Juntendo University Nerima Hospital, 3-1-10 Takanodai, Nerima-ku, Tokyo 177-8521, Japan; Department of Obstetrics and Gynecology, Juntendo University Faculty of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo 113-8421, Japan.
⁴ Department of Microbiology, Meiji Pharmaceutical University, 2-522-1, Noshio, Kiyose, Tokyo 204-8588, Japan. Electronic address: sugita@my-pharm.ac.jp.

PMID: 30682384
DOI: 10.1016/j.mimet.2019.01.011

Abstract

A rapid detection method for Lactobacillus crispatus and Lactobacillus iners, which are important for maintaining a healthy vaginal environment, was developed using loop-mediated isothermal amplification (LAMP). The LAMP assay had a lower limit of detection of 10 fg DNA and could detect both species within 45 min.

Keywords: Bacterial vaginosis; LAMP; Lactobacillus crispatus; Lactobacillus iners; Loop-mediated isothermal amplification; Rapid detection.

PubMed Disclaimer

Publication types

Actions

MeSH terms

Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions

Substances

Actions
Actions

LinkOut - more resources

Full Text Sources
- ClinicalKey
- Elsevier Science
Other Literature Sources
- The Lens - Patent Citations Database

Save citation to file

Email citation

Add to Collections

Add to My Bibliography

Your saved search

Create a file for external citation management software

Your RSS Feed

Rapid detection of Lactobacillus crispatus and Lactobacillus iners in vaginal specimens by loop-mediated isothermal amplification

Affiliations

Rapid detection of Lactobacillus crispatus and Lactobacillus iners in vaginal specimens by loop-mediated isothermal amplification

Authors

Affiliations

Abstract

Publication types

MeSH terms

Substances

LinkOut - more resources

Full Text Sources

Other Literature Sources