Online Analysis of Drug Toxicity to Cells with Shear Stress on an Integrated Microfluidic Chip

Abstract

Mechanical stimulation, especially fluid shear stress (FSS), is essential for a cell to regulate regular behaviors. A high-throughput platform to provide varying FSS for cell research is desperately required for better mimicking of the complex fluidic microenvironment. This work reports an integrated microfluidic chip that could afford five different FSS gradients consistently to investigate drug toxicity on cells with the stimulation of FSS. Compared with traditional methods to provide FSS, this device would be easier to operate, have higher throughput, and could eliminate interference factors from the culture environment for cells (apart from the unique variable FSS). On such a multi-FSS platform, effects of drugs toxicity on cells were exhibited, which would be more intense than that under static conditions. The results indicated that FSS enhanced the drug toxicity. The designed biochip provides an easy and high-throughput platform to evaluate the toxicity of drugs in the more authentic microenvironment and could be promisingly applied in future drug screening tests.

Keywords: Vandetanib; drug toxicity; endothelial cells; fluid shear stress; microfluidic chip.