Pharmacosynthetic Deconstruction of Sleep-Wake Circuits in the Brain

Abstract

Over the past decade, basic sleep research investigating the circuitry controlling sleep and wakefulness has been boosted by pharmacosynthetic approaches, including chemogenetic techniques using designed receptors exclusively activated by designer drugs (DREADD). DREADD offers a series of tools that selectively control neuronal activity as a way to probe causal relationship between neuronal sub-populations and the regulation of the sleep-wake cycle. Following the path opened by optogenetics, DREADD tools applied to discrete neuronal sub-populations in numerous brain areas quickly made their contribution to the discovery and the expansion of our understanding of critical brain structures involved in a wide variety of behaviors and in the control of vigilance state architecture.

Keywords: Arousal; Chemogenetic; Designer Receptor Exclusively Activated by Designer Drugs (DREADD); Non-REM sleep; Paradoxical sleep; Pharmacogenetic; REM sleep; Slow-wave sleep; Wakefulness.