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. 2019 Jan 14:9:3254.
doi: 10.3389/fmicb.2018.03254. eCollection 2018.

Distribution of ExPEC Virulence Factors, bla CTX-M, fos A3, and mcr-1 in Escherichia coli Isolated From Commercialized Chicken Carcasses

Paula Signolfi Cyoia  1 , Vanessa Lumi Koga  1 , Erick Kenji Nishio  1 , Sébastien Houle  2 , Charles M Dozois  2 , Kelly Cristina Tagliari de Brito  3 , Benito Guimarães de Brito  3 , Gerson Nakazato  1 , Renata Katsuko Takayama Kobayashi  1
Affiliations

Distribution of ExPEC Virulence Factors, bla CTX-M, fos A3, and mcr-1 in Escherichia coli Isolated From Commercialized Chicken Carcasses

Paula Signolfi Cyoia et al. Front Microbiol. .

Abstract

Pathogenic Escherichia coli found in humans and poultry carcasses harbor similar virulence and resistance genes. The present study aimed to analyze the distribution of extraintestinal pathogenic E. coli (ExPEC) virulence factors (VF), bla CTX-M groups, fosA3, and mcr-1 genes in E. coli isolated from commercialized chicken carcasses in southern Brazil and to evaluate their pathogenic risk. A total of 409 E. coli strains were isolated and characterized for genes encoding virulence factors described in ExPEC. Results of antimicrobial susceptibility testing confirmed that the strains were resistant to β-lactams, fosfomycin, colistin, and others resistance groups. The highest prevalence of VFs was observed in isolates belonging to the CTX-M groups, especially the CTX-M-2 group, when compared to those in other susceptible strains or strains with different mechanisms of resistance. Furthermore, ESBL strains were found to be 1.40 times more likely to contain three to five ExPEC virulence genes than non-ESBL strains. Our findings revealed the successful conjugation between ESBL-producing E. coli isolated from chicken carcass and the E. coli recipient strain J53, which suggested that genetic determinants encoding CTX-M enzymes may have originated from animals and could be transmitted to humans via food chain. In summary, chicken meat is a potential reservoir of MDR E. coli strains harboring resistance and virulence genes that could pose serious risks to human public health.

Keywords: CTX-M; ESBL; fosfomycin; multidrug-resistance; phylogenetic groups.

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Figures

Figure 1
Figure 1
Percentage resistance exhibited by ESBL-producing E. coli strains and non-ESBL-producing E. coli strains isolated from commercial chicken carcasses in southern Brazil from 2013 to 2014. *p < 0.05 by Pearson's Chi-square test.
Figure 2
Figure 2
Distribution of ESBL genes encoding CTX-Ms, TEM, and SHV detected in E. coli strains isolated from commercial chicken carcasses in southern Brazil from 2013 to 2014.
See this image and copyright information in PMC

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