[Determination of N-nitrosodiphenylamine in meat canned food for children by the method of chromato-massspectrometry]

Abstract

This study demonstrates the results obtained from the GC/MS experimental determination of low concentrations of N-nitrosodiphenylamine in meat canned baby food in the concentration range of 0.027-3.89 mg/kg. The perfect conditions of sample preparation (extraction with organic solvent and solid phase extraction) as well as the application of the chromatography-mass spectrometry allowed us to detect N-nitrosodiphenylamine in samples of the meat canned baby food with high selectivity in concentrations ranged from 0.016 to 5 mg/kg when an error of 23% was assumed. The use of the reaction of transesterification of fatty acids by potassium methylate, the removal of the ester generated from the samples of canned meat by organic solvent (hexane), concentrating of N-nitrosodiphenylamine in the aqueous layer on the cartridges of an automatic solid-phase extraction system provided 99.94% extraction of N-nitrosodiphenylamine from the canned meat samples. The experiment has made evident the presence of N-nitrosodiphenylamine in the samples of canned meat (beef + chicken) with the help of mass-spectrometry method in the mode of full ion scanning using the AMDIS automatic mass-spectral identification system.

Keywords: N-nitrosodiphenylamine; baby food; canned meat; chromatography-mass spectrometry; solid phase extraction.