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. 2019 Jul;41(7):2239-2248.
doi: 10.1002/hed.25689. Epub 2019 Feb 1.

Aurora kinase A induces chemotherapy resistance through revival of dormant cells in laryngeal squamous cell carcinoma

Li-Yun Yang  1 , Ya-Min Shan  2 , Yi Zhang  1 , En-Hui Zhou  1 , Xiao-Ping Chen  1 , Hao Zhang  2
Affiliations

Aurora kinase A induces chemotherapy resistance through revival of dormant cells in laryngeal squamous cell carcinoma

Li-Yun Yang et al. Head Neck. 2019 Jul.

Erratum in

  • ERRATUM.
    [No authors listed] [No authors listed] Head Neck. 2019 Aug;41(8):2839. doi: 10.1002/hed.25860. Head Neck. 2019. PMID: 31294903 No abstract available.

Abstract

Background: Chemotherapy resistance was an important tumor metastasis mechanism.

Methods: Cell Counting Kit-8 assay and plate colony formation assay were applied to examine the proliferation of laryngeal squamous cell carcinoma (LSCC). Immunofluorescent staining and Western blotting were carried out to show the expression of related proteins. Wound healing, migration, and invasion assays were used to examine the mobility, migration, and invasion of LSCC.

Results: Downregulated Aurora kinase A (AURKA) increased chemotherapy sensitivity and reduced the ability of mobility, migration, and invasion of Hep2 cells, while upregulated AURKA possessed opposite results. Hep2/5-Fu cells possessed dormancy-like properties and upregulated AURKA in Hep2/5-Fu cells (Hep2/5-Fu/AURKA cells) revived dormant state. Furthermore, Erk1/2 was restrained in Hep2/5-Fu cells and activated in Hep2/5-Fu/AURKA cells. Moreover, Erk1/2 accelerated the ability of mobility, migration, and invasion in Hep2/5-Fu/AURKA cells.

Conclusion: AURKA activated dormant state to induce chemotherapy resistance and promoted metastasis of LSCC through Erk1/2 pathway.

Keywords: 5-Fu resistance; Erk1/2; LSCC; aurora kinase A; dormancy.

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