Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2019 Feb 2;24(3):547.
doi: 10.3390/molecules24030547.

Optimal Extraction Study of Gastrodin-Type Components from Gastrodia Elata Tubers by Response Surface Design with Integrated Phytochemical and Bioactivity Evaluation

Minhui Hu  1 , Hui Yan  2 , Yuanyuan Fu  3 , Yulan Jiang  4 , Weifeng Yao  5 , Sheng Yu  6 , Li Zhang  7 , Qinan Wu  8 , Anwei Ding  9 , Mingqiu Shan  10
Affiliations

Optimal Extraction Study of Gastrodin-Type Components from Gastrodia Elata Tubers by Response Surface Design with Integrated Phytochemical and Bioactivity Evaluation

Minhui Hu et al. Molecules. .

Abstract

Gastrodia elata tuber (GET) is a popular traditional Chinese medicines (TCMs). In this study, response surface methodology (RSM) with a Box⁻Behnken design (BBD) was performed to optimize the extraction parameters of gastrodin-type components (gastrodin, gastrodigenin, parishin A, parishin B, parishin C and parishin E). Different from the conventional studies that merely focused on the contents of phytochemical, we gave consideration to both quantitative analysis of the above six components by HPLC and representative bioactivities of GET, including antioxidation and protection of human umbilical vein endothelial cells (HUVEC). Four independent variables (ethanol concentration, liquid-material ratio, soaking time and extraction time) were investigated with the integrated evaluation index of phytochemical contents. With the validation experiments, the optimal extraction parameters were as follows: ethanol concentration of 41%, liquid⁻solid ratio of 28.58 mL/g, soaking time of 23.91 h and extraction time of 46.60 min. Under the optimum conditions, the actual standardized comprehensive score was 1.8134 ± 0.0110, which was in accordance with the predicted score of 1.8100. This firstly established method was proved to be feasible and reliable to optimize the extraction parameters of the bioactive components from GET. Furthermore, it provides some reference for the quality control and extraction optimization of TCMs.

Keywords: Antioxidation; Gastrodia elata tuber (GET); Gastrodin-type components; HUVEC; Response surface methodology.

PubMed Disclaimer

Conflict of interest statement

The authors have declared no conflict of interest.

Figures

Figure 1
Figure 1
Chemical structures of the gastrodin-type components.
Figure 2
Figure 2
HPLC chromatograms of gastrodin-type components in (A) standard solution and (B) sample solution.
Figure 3
Figure 3
Effect of different extraction parameters (A: ethanol concentration, %; B: liquid-solid ratio, mL/g; C: extraction time, min; D: soaking time, h) on Y1.
Figure 4
Figure 4
The single-factor study of DPPH assay (A: reaction time B: DPPH concentration).
Figure 5
Figure 5
Response surface showing the interaction effects of different parameters (A: X1 and X2; B: X1 and X3; C: X1 and X4; D: X2 and X3; E: X2 and X4; F: X3 and X4) on the response Y (X1: ethanol concentration, %; X2: liquid-solid ratio, mL/g; X3: extraction time, min; X4: soaking time, h).
See this image and copyright information in PMC

References

    1. Lee D.K., Lim D.K., Um J.A., Lim C.J., Hong J.Y., Yoon Y.A., Ryu Y., Kim H.J., Cho H.J., Park J.H., et al. Evaluation of four different analytical tools to determine the regional origin of Gastrodia elata and Rehmannia glutinosa on the basis of metabolomics study. Molecules. 2014;19:6294–6308. doi: 10.3390/molecules19056294. - DOI - PMC - PubMed
    1. The National Pharmacopoeia Commission of P.R. China . Pharmacopoeia of the People’s Republic of China. China Medicine Science Technology Press; Beijing, China: 2015. pp. 200–201.
    1. Song E., Chung H., Shim E., Jeong J.K., Han B.K., Choi H.J., Hwang J. Gastrodia elata Blume extract modulates antioxidant activity and ultraviolet A-irradiated skin aging in human dermal fibroblast cells. J. Med. Food. 2016;19:1057–1064. doi: 10.1089/jmf.2016.3722. - DOI - PubMed
    1. Ahn E.K., Jeon H.J., Lim E.J., Jung H.J., Park E.H. Anti-inflammatory and anti-angiogenic activities of Gastrodia elata Blume. J. Ethnopharmacol. 2007;110:476–482. doi: 10.1016/j.jep.2006.10.006. - DOI - PubMed
    1. Huang G.B., Zhao T., Muna S.S., Jin H.M., Park J.I., Jo K.S., Lee B.H., Chae S.W., Kim S.Y., Park S.H., et al. Therapeutic potential of Gastrodia elata Blume for the treatment of Alzheimer’s disease. Neural Regen. Res. 2013;8:1061–1070. - PMC - PubMed

MeSH terms