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Comment
. 2019 Feb 15;38(4):e101526.
doi: 10.15252/embj.2019101526. Epub 2019 Feb 4.

An airway organoid is forever

Margherita Paschini  1   2 , Carla F Kim  1   2   3
Affiliations
Comment

An airway organoid is forever

Margherita Paschini et al. EMBO J. .

Abstract

In the last decade, the generation and maintenance of organotypic structures has been propelled to the center stage of biomedical research. In the lung, a variety of protocols has been devised to generate organoids mimicking lung structures, but most methods with human cells have complicated lengthy protocols or a progressive decline in differentiation potential and physiological function with increasing passaging. A new study from Sachs et al (2019) seeks to solve these issues, providing a versatile methodology to efficiently isolate, indefinitely culture, and manipulate human airway organoids, potentially allowing the in vitro modeling of a plethora of lung diseases.

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Figures

Figure 1
Figure 1. Long‐term cultivation of human airway organoids for disease modeling
A standardized and feasible method to indefinitely propagate and differentiate lung epithelium from human samples allows for in‐depth investigation of the biology of the human lung in health and disease states.
See this image and copyright information in PMC

Comment on

  • Long-term expanding human airway organoids for disease modeling.
    Sachs N, Papaspyropoulos A, Zomer-van Ommen DD, Heo I, Böttinger L, Klay D, Weeber F, Huelsz-Prince G, Iakobachvili N, Amatngalim GD, de Ligt J, van Hoeck A, Proost N, Viveen MC, Lyubimova A, Teeven L, Derakhshan S, Korving J, Begthel H, Dekkers JF, Kumawat K, Ramos E, van Oosterhout MF, Offerhaus GJ, Wiener DJ, Olimpio EP, Dijkstra KK, Smit EF, van der Linden M, Jaksani S, van de Ven M, Jonkers J, Rios AC, Voest EE, van Moorsel CH, van der Ent CK, Cuppen E, van Oudenaarden A, Coenjaerts FE, Meyaard L, Bont LJ, Peters PJ, Tans SJ, van Zon JS, Boj SF, Vries RG, Beekman JM, Clevers H. Sachs N, et al. EMBO J. 2019 Feb 15;38(4):e100300. doi: 10.15252/embj.2018100300. Epub 2019 Jan 14. EMBO J. 2019. PMID: 30643021 Free PMC article.

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