Resistance gene cloning from a wild crop relative by sequence capture and association genetics

Sanu Arora¹, Burkhard Steuernagel¹, Kumar Gaurav¹, Sutha Chandramohan², Yunming Long³, Oadi Matny⁴, Ryan Johnson⁴, Jacob Enk⁵, Sambasivam Periyannan², Narinder Singh⁶, M Asyraf Md Hatta^{1

7}, Naveenkumar Athiyannan^{2

8}, Jitender Cheema¹, Guotai Yu¹, Ngonidzashe Kangara¹, Sreya Ghosh¹, Les J Szabo⁹, Jesse Poland⁶, Harbans Bariana¹⁰, Jonathan D G Jones¹¹, Alison R Bentley¹², Mick Ayliffe², Eric Olson¹³, Steven S Xu¹⁴, Brian J Steffenson⁴, Evans Lagudah², Brande B H Wulff¹⁵

Affiliations

¹ John Innes Centre, Norwich Research Park, Norwich, UK.
² Commonwealth Scientific and Industrial Research Organization Agriculture and Food, Canberra, Australian Capital Territory, Australia.
³ Department of Plant Sciences, North Dakota State University, Fargo, ND, USA.
⁴ Department of Plant Pathology, University of Minnesota, St. Paul, MN, USA.
⁵ Arbor Biosciences, Ann Arbor, MI, USA.
⁶ Wheat Genetics Resource Center, Department of Plant Pathology, Kansas State University, Manhattan, KS, USA.
⁷ Faculty of Agriculture, Universiti Putra Malaysia, Serdang, Malaysia.
⁸ Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, Brisbane, Queensland, Australia.
⁹ US Department of Agriculture, Agriculture Research Service, Cereal Disease Laboratory, St. Paul, MN, USA.
¹⁰ The University of Sydney Plant Breeding Institute, Cobbitty, New South Wales, Australia.
¹¹ The Sainsbury Laboratory, Norwich Research Park, Norwich, UK.
¹² The John Bingham Laboratory, NIAB, Cambridge, UK.
¹³ Michigan State University, East Lansing, MI, USA.
¹⁴ US Department of Agriculture, Agriculture Research Service, Northern Crop Science Laboratory, Cereal Crops Research Unit, Red River Valley Agricultural Research Center, Fargo, ND, USA.
¹⁵ John Innes Centre, Norwich Research Park, Norwich, UK. brande.wulff@jic.ac.uk.

PMID: 30718880
DOI: 10.1038/s41587-018-0007-9

Resistance gene cloning from a wild crop relative by sequence capture and association genetics

Sanu Arora et al. Nat Biotechnol. 2019 Feb.

. 2019 Feb;37(2):139-143.

doi: 10.1038/s41587-018-0007-9. Epub 2019 Feb 4.

Authors

Affiliations

¹ John Innes Centre, Norwich Research Park, Norwich, UK.
² Commonwealth Scientific and Industrial Research Organization Agriculture and Food, Canberra, Australian Capital Territory, Australia.
³ Department of Plant Sciences, North Dakota State University, Fargo, ND, USA.
⁴ Department of Plant Pathology, University of Minnesota, St. Paul, MN, USA.
⁵ Arbor Biosciences, Ann Arbor, MI, USA.
⁶ Wheat Genetics Resource Center, Department of Plant Pathology, Kansas State University, Manhattan, KS, USA.
⁷ Faculty of Agriculture, Universiti Putra Malaysia, Serdang, Malaysia.
⁸ Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, Brisbane, Queensland, Australia.
⁹ US Department of Agriculture, Agriculture Research Service, Cereal Disease Laboratory, St. Paul, MN, USA.
¹⁰ The University of Sydney Plant Breeding Institute, Cobbitty, New South Wales, Australia.
¹¹ The Sainsbury Laboratory, Norwich Research Park, Norwich, UK.
¹² The John Bingham Laboratory, NIAB, Cambridge, UK.
¹³ Michigan State University, East Lansing, MI, USA.
¹⁴ US Department of Agriculture, Agriculture Research Service, Northern Crop Science Laboratory, Cereal Crops Research Unit, Red River Valley Agricultural Research Center, Fargo, ND, USA.
¹⁵ John Innes Centre, Norwich Research Park, Norwich, UK. brande.wulff@jic.ac.uk.

PMID: 30718880
DOI: 10.1038/s41587-018-0007-9

Abstract

Disease resistance (R) genes from wild relatives could be used to engineer broad-spectrum resistance in domesticated crops. We combined association genetics with R gene enrichment sequencing (AgRenSeq) to exploit pan-genome variation in wild diploid wheat and rapidly clone four stem rust resistance genes. AgRenSeq enables R gene cloning in any crop that has a diverse germplasm panel.

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Comment in

A new mapping strategy.
Lyu J. Lyu J. Nat Plants. 2019 Mar;5(3):245. doi: 10.1038/s41477-019-0391-2. Nat Plants. 2019. PMID: 30850812 No abstract available.

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Resistance gene cloning from a wild crop relative by sequence capture and association genetics

Affiliations

Resistance gene cloning from a wild crop relative by sequence capture and association genetics

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Abstract

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