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. 2019 Jun 15:283:338-344.
doi: 10.1016/j.foodchem.2018.12.128. Epub 2019 Jan 14.

Ultrasensitive detection of staphylococcal enterotoxin B in foodstuff through dual signal amplification by bio-barcode and real-time PCR

Yanyang Xu  1 , Bingyang Huo  2 , Chao Li  3 , Yuan Peng  3 , Sai Tian  4 , Longxing Fan  5 , Jialei Bai  3 , Baoan Ning  3 , Zhixian Gao  6
Affiliations

Ultrasensitive detection of staphylococcal enterotoxin B in foodstuff through dual signal amplification by bio-barcode and real-time PCR

Yanyang Xu et al. Food Chem. .

Abstract

This study developed an ultrasensitive method involving (1) gold nanoparticles encoded with a double-stranded DNA as the first signal amplification and goat anti-staphylococcal enterotoxin B (SEB) polyclonal antibody and (2) magnetic microparticles coated with anti-SEB monoclonal antibody to detect SEB. Both functionalized nanoparticles can capture SEB in a sandwich system. The released DNA barcodes were then characterized through SYBR Green real-time polymerase chain reaction and resulted in the second signal amplification. Under optimized experimental conditions, an ultrasensitive bio-barcode for SEB detection was established, and its detection limit could reach 0.269 pg mL-1, which was 1000-fold lower than that of conventional enzyme-linked/immunosorbent assay. The proposed method shows great potential in food security.

Keywords: Bio-barcode assay; Real-time PCR; Staphylococcal enterotoxin B.

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