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. 2019 Jul;74(7):1381-1384.
doi: 10.1111/all.13734. Epub 2019 Mar 11.

Soluble FcɛRI: A biomarker for IgE-mediated diseases

Sherezade Moñino-Romero  1   2 , Willem S Lexmond  2   3 , Josef Singer  2   4   5   6 , Christina Bannert  1 , Abena S Amoah  7 , Maria Yazdanbakhsh  7 , Daniel A Boakye  8 , Erika Jensen-Jarolim  4   5 , Edda Fiebiger  2   3 , Zsolt Szépfalusi  1
Affiliations

Soluble FcɛRI: A biomarker for IgE-mediated diseases

Sherezade Moñino-Romero et al. Allergy. 2019 Jul.
No abstract available

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Conflict of interest statement

The authors declare that they have no conflicts of interest.

Figures

Figure 1
Figure 1
sFcεRI is highly expressed in allergic individuals and it is modulated by allergen exposure. Detection of total and IgE‐bound sFcεRI levels by ELISA. Total sFcεRI levels in control and atopic (n = 148) groups (A). Correlation between total sFcεRI and total IgE levels in atopic group (B). Total and IgE‐bound sFcεRI levels in atopic group (C). Total sFcεRI levels in control and IgE diseases groups (D). Total sFcεRI levels with and without sIgE sensitizations, and normal and elevated IgE levels in AD (E‐F) and AA (G‐H). Total sFcεRI levels during OFC (I). Graphs represent individuals with median plus IQR. Mann‐Whitney test (A, E‐H), Kruskal‐Wallis test plus Dunn's multiple correction (C), and Spearman r coefficient ranks (B, D) were performed, where *P < 0.05, **P < 0.01, and ****P < 0.0001. Co: control (n = 17); IV: insect venom (n = 9); AD: atopic dermatitis (n = 45); AA: allergic asthma (n = 69); FA: food allergy (n = 59); Pos: positive; Neg: negative; IQR: interquartile range; OFC: oral food challenge (n = 13) [Colour figure can be viewed at wileyonlinelibrary.com]
Figure 2
Figure 2
sFcεRI interferes with IgE detection ELISA. Detection of IgE and sFcεRI levels by ELISA and Western Blot. Representation of rsFcεRI and rsFcεRI m proteins (A). Detection of rsFcεRI and rsFcεRI m proteins by Western Blot analysis in nonreducing and reducing conditions (B‐C). Detection of IgE pre‐incubated with rsFcεRI and rsFcεRI m proteins in a 500 ng/mL cIgE solution (D‐E). Detection of IgE pre‐incubated with rsFcεRI in human IgE (1:10‐1:100) or human serum (3202 and 903 ng/mL) solutions (F‐G). Graphs represent assay triplicates of a representative experiment (D‐E), or assay duplicates of biological triplicates (F) or two individuals (G). Spearman coefficient rank analysis or 1‐way ANOVA test plus Tukey's multiple correction was performed, where *P < 0.05 and ****P < 0.0001
See this image and copyright information in PMC

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