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. 1997 May;16(8):513-519.
doi: 10.1007/BF01142315.

A simple system for pea transformation

S J Bean  1 , P S Gooding  1 , P M Mullincaux  1 , D R Davies  1
Affiliations

A simple system for pea transformation

S J Bean et al. Plant Cell Rep. 1997 May.

Abstract

The lateral cotyledonary meristems of germinatingPisum sativum cv. Puget seeds were used to develop a reproducibleAgrobacterium tumefaciens-mediated transformation system. This procedure exhibits distinct advantages over those previously reported, in that it uses dry seed as starting material, and the highly regenerable cotyledonary meristems rapidly produce transgenic shoots without an intermediate callus phase. This transformation regime facilitates the rapid generation of phenotypically normal, self-fertile plants containing functional transgenes inherited in a Mendelian fashion.

Keywords: Agrobacterium tumefaciens; Bar gene; Cotyledonary meristem; Pea transformation; Phosphinothricin resistance.

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