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. 2019 Mar;59(1):90-95.
doi: 10.1007/s12088-018-0742-0. Epub 2018 May 18.

Mining the Core Gut Microbiome from a Sample Indian Population

Abhijit S Kulkarni  1 , Shreyas V Kumbhare  1 , Dhiraj P Dhotre  2 , Yogesh S Shouche  1   2
Affiliations

Mining the Core Gut Microbiome from a Sample Indian Population

Abhijit S Kulkarni et al. Indian J Microbiol. 2019 Mar.

Abstract

Human gut microbiome studies are increasing at a rapid pace to understand contributions of the prokaryotic life to the innate workings of their eukaryotic host. Majority of studies focus on the pattern of gut microbial diversity in various diseases, however, understanding the core microbiota of healthy individuals presents a unique opportunity to study the microbial fingerprint in population specific studies. Present study was undertaken to determine the core microbiome of a healthy population and its imputed metabolic role. A total of 8990, clone library sequences (> 900 bp) of 16S rRNA gene from fecal samples of 43 individuals were used. The core gut microbiota was computed using QIIME pipeline. Our results show the distinctive predominance of genus Prevotella and the core composition of genera Prevotella, Bacteroides, Roseburia and Megasphaera in the Indian gut. PICRUSt analysis for functional imputation of the microbiome indicates a higher potential of the microbiota for carbohydrate metabolism. The presence of core microbiota may indicate key functions played by these microbes for the human host.

Keywords: Core microbiome; Gut microbiota; Indian gut microbiome.

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Conflict of interest statement

Compliance with Ethical StandardsThe authors declare that they have no conflict of interest.

Figures

Fig. 1
Fig. 1
Genus-level distribution
Fig. 2
Fig. 2
Phylogenetic tree for taxonomic classification: Prevotella copri
Fig. 3
Fig. 3
a PICRUSt analysis showing potential of microbiome for various biochemical pathways. b PATRIC analysis indication percentage of genes present for each biochemical pathway
See this image and copyright information in PMC

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