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. 2018 Dec 31;62(4):511-517.
doi: 10.2478/jvetres-2018-0077. eCollection 2018 Dec.

Control of Residues of Thyreostats in Slaughter Animals in Poland in 2011-2017

Barbara Woźniak  1 , Iwona Matraszek-Żuchowska  1 , Katarzyna Sielska  1 , Sebastian Witek  1 , Andrzej Posyniak  1 , Krzysztof Niemczuk  1 , Jan Żmudzki  1
Affiliations

Control of Residues of Thyreostats in Slaughter Animals in Poland in 2011-2017

Barbara Woźniak et al. J Vet Res. .

Abstract

Introduction: In the European Union, the use of thyreostatic drugs for fattening slaughter animals has been banned since 1981 under Council Directive 81/602/EEC. For protection of consumer health against unwanted residues and in compliance with Directive 96/23, each EU country must monitor thyreostats in samples of animal origin. This paper presents the results of research on thyreostatic residues carried out in Poland in 2011-2017.

Material and methods: The material for testing was urine (n = 3,491), drinking water (n = 127), and muscle samples (n = 349) officially collected by Veterinary Sanitary Inspectors in slaughterhouses and farms throughout the country in accordance with the national residue control plan. The samples were examined for the presence of tapazole, thiouracil, methylthiouracil, propylthiouracil, and phenylthiouracil using liquid chromatography tandem mass spectrometry through an accredited method.

Results: In four bovine and three porcine urine samples, the permissible thiouracil concentration was exceeded. In one sample of porcine urine, methyl- and propylthiouracil were found. The presence of thiouracil and its derivatives in urine samples is most likely due to feeding animals diet containing cruciferous plants.

Conclusions: The results of research indicate that thyreostats are not used for anabolic purposes in slaughter animals in Poland.

Keywords: monitoring; thiouracil; thyreostats; urine.

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Conflict of interest statement

Conflict of Interests Statement: The authors declare that there is no conflict of interests regarding the publication of this article.

Figures

Fig. 1
Fig. 1
LC-MS/MS MRM chromatograms of a) a bovine urine sample spiked at 10 μg L−1 with TU; b) a real urine bovine sample confirmed at 40.4 ± 10.0 μg L−1 of thiouracil; c) a real urine sample from a pig confirmed at 6.5 μg L−1 of thiouracil, 5.1 ± 2.3 μg L−1 of methylthiouracil, and 3.4 ± 0.9 μg L−1 of propylthiouracil
See this image and copyright information in PMC

References

    1. Commission Decision 2002/657/EC of 12th August 2002 implementing Council Directive 96/23/EC concerning the performance of analytical methods and the interpretation of results. Off J Eur Commun 2002, L. 221:8–36.
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    1. CRL Guidance Paper. CRLs view on state of the art analytical methods for national residue control plans. 2007. http://www.rivm.nl/bibliotheek/digitaaldepot/crlguidance2007.pdf December.
    1. European Commission, Health & Consumers Directorate-General. Residue expert working group –. 2012. 27 April. 120427 Draft Minutes.
    1. European Community Council Directive 81/602/EEC concerning the prohibition of certain substances having a hormonal action and of any substances having a thyrostatic action. Off J Eur Communities1981, L. 222:32–33.

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