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Comment
. 2019 Feb 7;24(2):201-203.
doi: 10.1016/j.stem.2019.01.010.

Human Spermatogonial Stem Cells Scrutinized under the Single-Cell Magnifying Glass

Kun Tan  1 , Miles F Wilkinson  2
Affiliations
Comment

Human Spermatogonial Stem Cells Scrutinized under the Single-Cell Magnifying Glass

Kun Tan et al. Cell Stem Cell. .

Abstract

Spermatogonial stem cells (SSCs) are essential for adult spermatogenesis. Recently, Wang et al. (2018), Guo et al. (2018), and Hermann et al. (2018) used single-cell RNA sequencing to define and molecularly characterize human testicular cell populations, including spermatogonial subsets with characteristics of human SSCs.

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Conflict of interest statement

DECLARATION OF INTEREST

The authors declare no competing interests.

Figures

Figure 1.
Figure 1.
(A) Guo et al., Hermann et al., and Wang et al. performed scRNAseq analysis to identify human testicular germ and somatic cell subsets. (B) Top: cartoon of major spermatogonial cell types and their developmental relationships. Bottom: developmental expression pattern of marker genes, as well as enriched gene ontology (GO) functions, as determined by scRNAseq analysis.
See this image and copyright information in PMC

Comment on

  • Single-Cell RNA Sequencing Analysis Reveals Sequential Cell Fate Transition during Human Spermatogenesis.
    Wang M, Liu X, Chang G, Chen Y, An G, Yan L, Gao S, Xu Y, Cui Y, Dong J, Chen Y, Fan X, Hu Y, Song K, Zhu X, Gao Y, Yao Z, Bian S, Hou Y, Lu J, Wang R, Fan Y, Lian Y, Tang W, Wang Y, Liu J, Zhao L, Wang L, Liu Z, Yuan R, Shi Y, Hu B, Ren X, Tang F, Zhao XY, Qiao J. Wang M, et al. Cell Stem Cell. 2018 Oct 4;23(4):599-614.e4. doi: 10.1016/j.stem.2018.08.007. Epub 2018 Aug 30. Cell Stem Cell. 2018. PMID: 30174296
  • The adult human testis transcriptional cell atlas.
    Guo J, Grow EJ, Mlcochova H, Maher GJ, Lindskog C, Nie X, Guo Y, Takei Y, Yun J, Cai L, Kim R, Carrell DT, Goriely A, Hotaling JM, Cairns BR. Guo J, et al. Cell Res. 2018 Dec;28(12):1141-1157. doi: 10.1038/s41422-018-0099-2. Epub 2018 Oct 12. Cell Res. 2018. PMID: 30315278 Free PMC article.
  • The Mammalian Spermatogenesis Single-Cell Transcriptome, from Spermatogonial Stem Cells to Spermatids.
    Hermann BP, Cheng K, Singh A, Roa-De La Cruz L, Mutoji KN, Chen IC, Gildersleeve H, Lehle JD, Mayo M, Westernströer B, Law NC, Oatley MJ, Velte EK, Niedenberger BA, Fritze D, Silber S, Geyer CB, Oatley JM, McCarrey JR. Hermann BP, et al. Cell Rep. 2018 Nov 6;25(6):1650-1667.e8. doi: 10.1016/j.celrep.2018.10.026. Cell Rep. 2018. PMID: 30404016 Free PMC article.

References

    1. Di Persio S, Saracino R, Fera S, Muciaccia B, Esposito V, Boitani C, Berloco BP, Nudo F, Spadetta G, Stefanini M, et al. (2017). Spermatogonial kinetics in humans. Development 144, 3430–3439. - PubMed
    1. Fayomi AP, and Orwig KE (2018). Spermatogonial stem cells and spermatogenesis in mice, monkeys and men. Stem Cell Res 29, 207–214. - PMC - PubMed
    1. Green CD, Ma Q, Manske GL, Shami AN, Zheng X, Marini S, Moritz L, Sultan C, Gurczynski SJ, Moore BB, et al. (2018). A Comprehensive Roadmap of Murine Spermatogenesis Defined by Single-Cell RNA-Seq. Dev Cell 46, 651–667 e610. - PMC - PubMed
    1. Guo J, Grow EJ, Mlcochova H, Maher GJ, Lindskog C, Nie X, Guo Y, Takei Y, Yun J, Cai L, et al. (2018). The adult human testis transcriptional cell atlas. Cell Res - PMC - PubMed
    1. Guo J, Grow EJ, Yi C, Mlcochova H, Maher GJ, Lindskog C, Murphy PJ, Wike CL, Carrell DT, Goriely A, et al. (2017). Chromatin and Single-Cell RNA-Seq Profiling Reveal Dynamic Signaling and Metabolic Transitions during Human Spermatogonial Stem Cell Development. Cell Stem Cell 21, 533–546 e536. - PMC - PubMed