Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2019 Feb 8;19(1):19.
doi: 10.1186/s12902-019-0350-y.

The expression of sirtuins, superoxide dismutase, and lipid peroxidation status in peripheral blood from patients with diabetes and hypothyroidism

Abdullah Al-Khaldi  1 , Samar Sultan  2
Affiliations

The expression of sirtuins, superoxide dismutase, and lipid peroxidation status in peripheral blood from patients with diabetes and hypothyroidism

Abdullah Al-Khaldi et al. BMC Endocr Disord. .

Abstract

Background: Sirtuin 1 (SIRT1) and sirtuin 3 (SIRT3) proteins have an important role in counteracting oxidative stress. Although diabetes and hypothyroidism (HT) are both characterized by oxidative stress, the mechanisms are not fully understood. This study investigated the effects of type 1 diabetes (T1D), type 2 diabetes (T2D), and HT on the expression levels of SIRT1, SIRT3, and manganese superoxide dismutase (SOD2).

Methods: Gene expression of SIRT1, SIRT3, and SOD2 was measured using real-time PCR. The protein expression of SOD2 and lipid peroxidation (thiobarbituric acid reactive substances) was measured by the TBARS Assay kit and enzyme-linked immunosorbent assay (ELISA) respectively.

Results: The results showed that the SIRT1 and SIRT3 levels were lower in peripheral blood samples from patients with T1D, T2D, or HT than in healthy individuals. Interestingly, the mRNA and protein expression levels of SOD2 were higher in all three patient groups. Lipid peroxidation was higher in the patients with HT than in the healthy individuals.

Conclusions: These results indicate alterations in the expression levels of sirtuins and superoxide dismutase in diabetes and HT, which may be related, at least in part, to the oxidative stress. Identifying such alterations in those patients will pave the way towards the development of drugs to enhance SIRT1 and SIRT3 expression and their activity to prevent the damaging effect of oxidative stress.

Keywords: Hypothyroidism; Oxidative stress; Sirtuins; Diabetes; Superoxide dismutase.

PubMed Disclaimer

Conflict of interest statement

Ethics approval and consent to participate

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. Approval of the study was given by the Ethics Committee of the King Fahad Armed Forces Hospital, Jeddah, Saudi Arabia. Informed written consent was obtained from all individual participants included in the study.

Consent for publication

Not applicable.

Competing interests

The authors declare that they have no competing interests.

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Figures

Fig. 1
Fig. 1
Expression levels of SIRT1 in patients with T2D, T1D, HT and control individuals. The evaluation of the expression levels of SIRT1 and β-actin are shown for control (n = 13), T2D (n = 22), T1D (n = 5), and HT (n = 7). SIRT1 expression was measured by real-time PCR. Data are presented as means ± SEM. n donated the number of donors. *P < 0.05, **p < 0.01 versus controls
Fig. 2
Fig. 2
Expression levels of SIRT3 in patients with T2D, T1D, HT and control individuals. The evaluation of the expression levels of SIRT3 and β-actin are shown for control (n = 13), T2D (n = 22), T1D (n = 5), and HT (n = 7). SIRT3 expression was measured by real-time PCR. Data are presented as means ± SEM. n donated the number of donors. *p < 0.05, ***p < 0.001 versus controls
Fig. 3
Fig. 3
Expression levels of SOD2 in patients with T2D, T1D, HT, and control individuals. The evaluation of the expression levels of SOD2 and β-actin are shown for control (n = 13), T2D (n = 22), T1D (n = 5), and HT (n = 7). SOD2 expression was measured by real-time PCR. Data are presented as means ± SEM. n donated the number of donors. *p < 0.05, **p < 0.01, ***p < 0.001 versus controls
Fig. 4
Fig. 4
Serum concentrations of SOD2 in T2D, T1D, and HT. Secretion of SOD2 was measured by ELISA in control (n = 6), T2D (n = 4), T1D (n = 4) and HT (n = 4) (B). Data are presented as means ± SEM. n donated the number of donors*p < 0.05, **p < 0.01 versus controls
See this image and copyright information in PMC

References

    1. Merksamer PI, Liu Y, He W, Hirschey MD, Chen D, Verdin E. The sirtuins, oxidative stress and aging: an emerging link. Aging (Albany NY) 2013;5(3):144–150. - PMC - PubMed
    1. Ungvari Z, Labinskyy N, Mukhopadhyay P, Pinto JT, Bagi Z, Ballabh P, Zhang C, Pacher P, Csiszar A. Resveratrol attenuates mitochondrial oxidative stress in coronary arterial endothelial cells. Am J Physiol Heart Circ Physiol. 2009;297(5):H1876–H1881. - PMC - PubMed
    1. Gencoglu H, Tuzcu M, Hayirli A, Sahin K. Protective effects of resveratrol against streptozotocin-induced diabetes in rats by modulation of visfatin/sirtuin-1 pathway and glucose transporters. Int J Food Sci Nutr. 2015;66(3):314–320. - PubMed
    1. Price NL, Gomes AP, Ling AJY, Duarte FV, Martin-Montalvo A, North BJ, Agarwal B, Ye L, Ramadori G, Teodoro JS. SIRT1 is required for AMPK activation and the beneficial effects of resveratrol on mitochondrial function. Cell Metab. 2012;15(5):675–690. - PMC - PubMed
    1. Sun C, Zhang F, Ge X, Yan T, Chen X, Shi X, Zhai Q. SIRT1 improves insulin sensitivity under insulin-resistant conditions by repressing PTP1B. Cell Metab. 2007;6(4):307–319. - PubMed