Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2019:1920:183-218.
doi: 10.1007/978-1-4939-9009-2_12.

Detection of Gene and Protein Expression in Mouse Embryos and Tissue Sections

Edwina McGlinn  1 , Miriam A Holzman  2 , Jennifer H Mansfield  3
Affiliations

Detection of Gene and Protein Expression in Mouse Embryos and Tissue Sections

Edwina McGlinn et al. Methods Mol Biol. 2019.

Abstract

Analysis of gene (mRNA and protein) expression patterns is central to the study of embryonic development. This chapter details methods for detecting mRNA and protein expression in whole-mouse embryos and in tissue sections, including mRNA in situ hybridization, immunohistochemistry, and detection of enzymatic and fluorescent protein reporters. We focus on histological methods; molecular methods of measuring gene expression (for example, RNAseq, PCR) are not included here.

Keywords: Alkaline phosphatase; Green fluorescent protein; Immunohistochemistry; In situ hybridization; Mouse embryogenesis; ß-Galactosidase.

PubMed Disclaimer

Publication types

LinkOut - more resources