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. 2019:1923:227-241.
doi: 10.1007/978-1-4939-9024-5_10.

Tailoring N-Glycan Biosynthesis for Production of Therapeutic Proteins in Saccharomyces cerevisiae

Mari A Piirainen  1 , Heidi Salminen  1 , Alexander D Frey  2
Affiliations

Tailoring N-Glycan Biosynthesis for Production of Therapeutic Proteins in Saccharomyces cerevisiae

Mari A Piirainen et al. Methods Mol Biol. 2019.

Abstract

The ability to control and adjust the N-glycosylation pathway of Saccharomyces cerevisiae is a key step toward production of therapeutic glycoproteins such as antibodies or erythropoietin. The focus of this chapter is to describe the road from yeast-type N-glycosylation to human-type complex N-glycosylation. The chapter describes the cell engineering and provides the detailed analytical procedures required to perform glycan analysis using MALDI-TOF mass spectrometry.

Keywords: Glycan analysis; Glycoengineering; Glycosylation efficiency; Glycosyltransferases; MALDI-TOF MS; S. cerevisiae.

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