doi: 10.1016/0378-1119(88)90251-x.
Cloning the FnuDI, NaeI, NcoI and XbaI restriction-modification systems
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- PMID: 3074019
- DOI: 10.1016/0378-1119(88)90251-x
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Cloning the FnuDI, NaeI, NcoI and XbaI restriction-modification systems
Gene.
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Abstract
Methyltransferase genes from the FnuDI, NaeI, NcoI, and XbaI restriction-modification systems have been isolated in Escherichia coli by 'shot-gun' cloning bacterial DNA fragments into plasmid vectors and selecting for protectively modified molecules that resist digestion by the corresponding restriction endonuclease.
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