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. 1988 Dec 25;74(1):55-9.
doi: 10.1016/0378-1119(88)90251-x.

Cloning the FnuDI, NaeI, NcoI and XbaI restriction-modification systems

E M Van Cott  1 , G G Wilson
Affiliations

Cloning the FnuDI, NaeI, NcoI and XbaI restriction-modification systems

E M Van Cott et al. Gene. .

Abstract

Methyltransferase genes from the FnuDI, NaeI, NcoI, and XbaI restriction-modification systems have been isolated in Escherichia coli by 'shot-gun' cloning bacterial DNA fragments into plasmid vectors and selecting for protectively modified molecules that resist digestion by the corresponding restriction endonuclease.

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