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. 2018 Nov;40(1):680-686.
doi: 10.1080/0886022X.2018.1544565.

The anti-inflammatory effects of curcumin on renal ischemia-reperfusion injury in rats

Jiong Zhang  1 , Li Tang  2 , Gui Sen Li  1 , Jia Wang  3
Affiliations

The anti-inflammatory effects of curcumin on renal ischemia-reperfusion injury in rats

Jiong Zhang et al. Ren Fail. 2018 Nov.

Abstract

The present study aimed to explore the protective mechanism of curcumin-precondition in renal ischemia-reperfusion (I/R) injury. Thirty male SD rats were randomly equally divided into Sham, IRI, and Curcumin groups (n = 10). The model of IRI was induced by clamping the left renal artery for 45 min followed by 24 h reperfusion with the contralateral nephrectomy. ELISA was used to examine the expression of Cr, BUN, IL-8, TNF-α, and IL-6 in the serum; RT-PCR was used to detect the mRNA level of IL-8,TNF-α, and IL-6 in the kidney; The morphology of kidney was examined by Periodic Acid-Schiff stain (PAS). The expression of JAK2, p-JAK2, STAT3, p-STAT3, p65, and p-p65 in kidney were detected by Western blotting. The result displayed that Curcumin pretreatment can significantly increase the expression of p-JAK2 and p-STAT3 and reduce the expression of Cr, BUN, IL-8, TNF-α, IL-6, and p-p65. In addition, Curcumin can attenuate the kidney pathological injury and have no effect on the expression of JAK2, STAT3, and p65. Our findings showed the protective effect of Curcumin in I/R injury is associated with suppressing NF-κB mediating inflammation by activating JAK2/STAT3 signal pathway.

Keywords: Curcumin; inflammation; renal ischemia reperfusion injury.

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Figures

Figure 1.
Figure 1.
Effects of Curcumin pretreatment on renal function following renal I/R-induced injury. Serum creatinine and BUN were measured to assess the reno-protective effect of Cur against renal I/R. Data are represented as mean ± SEM (n = 10). ***p < .001 (IRI vs. Sham); #p < .005 (IRI vs. Cur).
Figure 2.
Figure 2.
Effects of Curcumin pretreatment on I/R-induced renal histology. Representative microphotographs were taken from the kidneys of the sham, IRI, and Cur groups at the time point of 24 h after renal I/R. Histopathological examination was performed using PAS staining. Semi-quantitative assessment of the histological lesions based on tubular necrosis (B). Data are represented as mean ± SEM (n = 10). ***p < .001 (IRI vs. Sham); #p < .001 (IRI vs. Cur).
Figure 3.
Figure 3.
Effects of Curcumin pretreatment on the expression of p65 and p-p65 after renal ischemia-reperfusion injury. Western blot analysis was employed to the expression of p65 and p-p65. (A) A representative result for Western blot analysis p65. (B) Semi-quantitative analysis of 10 animals studied in each group. The relative amounts of p-p65 and p65 in each group of rats were normalized by β-actin and presented as a ratio between p-p65 and p65. *p < .05 (IRI vs. Sham); #p < .05 (Cur vs. IRI).
Figure 4.
Figure 4.
Effects of Curcumin pretreatment on the JAK2/STAT3 signaling after renal ischemia-reperfusion injury. Western blot analysis was employed to the expression of JAK2, p-JAK2, STAT3, and p-STATA3. (A) A representative result for Western blot analysis p-JAK2, STAT3, and p-STATA3. (B) Semi-quantitative analysis of 10 animals studied in each group. The relative amounts of p-JAK2, JAK2, STAT3, and p-STATA3 in each group of rats were normalized by β-actin and presented as a ratio between p-JAK2/JAK2 and p-STAT3/STAT3. *p<.05 (IRI vs. Sham); #p<.05 (Curcumin vs. IRI). (IRI vs. Sham); #p<.05 (Cur vs. IRI).
Figure 5.
Figure 5.
The structure of curcumin.
See this image and copyright information in PMC

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