Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2019 Jan 1;15(2):416-429.
doi: 10.7150/ijbs.26884. eCollection 2019.

Small RNA sequencing reveals dynamic microRNA expression of important nutrient metabolism during development of Camellia oleifera fruit

Xiao-Xia Liu  1 , Xiao-Fang Luo  1 , Ke-Xin Luo  1 , Ya-Lin Liu  1 , Ting Pan  1 , Zhi-Zhang Li  1 , Gregory J Duns  1 , Fu-Lin He  1 , Zuo-Dong Qin  1
Affiliations

Small RNA sequencing reveals dynamic microRNA expression of important nutrient metabolism during development of Camellia oleifera fruit

Xiao-Xia Liu et al. Int J Biol Sci. .

Abstract

To obtain insight into the function of miRNAs in the synthesis and storage of important nutrients during the development of Camellia oleifera fruit, Illumina sequencing of flower and fruit small-RNA was conducted. The results revealed that 797 miRNAs were significantly differentially expressed between flower and fruit samples of Camellia oleifera. Through integrated GO and KEGG function annotations, it was determined that the miRNA target genes were mainly involved in metabolic pathways, plant hormone signal transduction, fruit development, mitosis and regulation of biosynthetic processes. Carbohydrate accumulation genes were differentially regulated by miR156, miR390 and miR395 in the fruit growth and development process. MiR477 is the key miRNA functioning in regulation of genes and involved in fatty acid synthesis. Additionally, miR156 also has the function of regulating glycolysis and nutrient transformation genes.

Keywords: Camellia oleifera; deeping sequencing; development of fruit; microRNA; nutrient metabolism regulation.

PubMed Disclaimer

Conflict of interest statement

Competing Interests: The authors have declared that no competing interest exists.

Figures

Figure 1
Figure 1
The features of Camellia oleifera fruit. (A) Fresh fruit. (B) Fresh seed. (C) SEM view of seed.
Figure 2
Figure 2
The fatty acid composition of camellia oil by GC-MS.
Figure 3
Figure 3
Length distribution of clean small RNAs in Camellia oleifera flower and fruit samples.
Figure 4
Figure 4
Distribution of significantly different expressed Camellia oleifera miRNAs in flower and fruit samples.
Figure 5
Figure 5
The plots of GO ontology enrichment in Camellia oleifera flower and fruit. (A) Biological process. (B) Cellular component. (C) Molecular function. Red to yellow colours represent decreasing significance levels (Red is most, yellow is least significant).
Figure 5
Figure 5
The plots of GO ontology enrichment in Camellia oleifera flower and fruit. (A) Biological process. (B) Cellular component. (C) Molecular function. Red to yellow colours represent decreasing significance levels (Red is most, yellow is least significant).
Figure 5
Figure 5
The plots of GO ontology enrichment in Camellia oleifera flower and fruit. (A) Biological process. (B) Cellular component. (C) Molecular function. Red to yellow colours represent decreasing significance levels (Red is most, yellow is least significant).
Figure 6
Figure 6
The top 20 statistics of pathway enrichment for fruit-VS-flower by Q value from small to large.
Figure 7
Figure 7
The KEGG pathways and related miRNAs as determined for Camellia oleifera. (A) Plant hormone signal transduction. (B) Photosynthesis.
Figure 7
Figure 7
The KEGG pathways and related miRNAs as determined for Camellia oleifera. (A) Plant hormone signal transduction. (B) Photosynthesis.
Figure 8
Figure 8
Validation of sequencing results by RT-qPCR. (A) MiRNAs. (B) Target genes. Error bars indicate mean ± SE (n = 3 per breed).
See this image and copyright information in PMC

References

    1. Zhuang R. Camellia oleifera in China. Beijing: China Forestry Press; 2008.
    1. Feng JL, Yang ZJ, Chen SP. et al. High throughput sequencing of small RNAs reveals dynamic microRNAs expression of lipid metabolism during Camellia oleifera and C. meiocarpa seed natural drying. BMC Genomics. 2017;18:546. - PMC - PubMed
    1. Xia EH, Jiang J, Huang H. et al. Transcriptome analysis of the oil-rich tea plant, Camellia oleifera, reveals candidate genes related to lipid metabolism. PLoS One. 2014;9:e104150. - PMC - PubMed
    1. Cheng YT, Lu C, Yen GC. Beneficial effects of camellia oil (Camellia oleifera Abel.) on hepatoprotective and gastroprotective activities. J Nutr Sci Vitaminol. 2015;61:S100–S2. - PubMed
    1. Dong B, Wu B, Hong W. et al. Transcriptome analysis of the tea oil camellia (Camellia oleifera) reveals candidate drought stress genes. PLoS One. 2017;12:e0181835. - PMC - PubMed

Publication types

MeSH terms