Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1988;55(222-223):167-70.

Changes in metaphasic chromosomal ultrastructure caused by denaturing treatments

E Cerdán  1 , J Pasantes, J Méndez, V Goyanes
Affiliations
  • PMID: 3075165

Changes in metaphasic chromosomal ultrastructure caused by denaturing treatments

E Cerdán et al. Cytobios. 1988.

Abstract

Denaturation and posterior renaturation of metaphase chromosomes after different treatments were tested by acridine orange staining. A relationship between conservation of ultrastructure and the capacity to renature was discovered. Amyl acetate, commonly used in electron microscopy to fix chromosomes, avoids the action of denaturing agents and this effect is not reversed by chromosome rehydration.

PubMed Disclaimer