Characterization of Curtoviruses Associated with Curly Top Disease of Tomato in California and Monitoring for These Viruses in Beet Leafhoppers

Abstract

Curly top disease is caused by a complex of curtoviruses (family Geminiviridae), and it continues to plague tomato production in California. To better understand the etiology of curly top of tomatoes in California, polymerase chain reaction (PCR)-based methods were developed and used to characterize the curtoviruses involved, and to monitor for these viruses in the beet leafhopper vector, Circulifer tenellus. From 2002 to 2008, 86 processing and fresh market tomato fields in the Central Valley of California were surveyed for the incidence of curly top symptoms. Representative samples with curly top symptoms were collected from the surveyed fields, as well as from another 24 fields. The incidence of curly top symptoms in most fields ranged from trace (<1%) to low (1 to 5%); however, in 2002, 2003, and 2008, some fields had medium (5 to 20%) or high (>20%) incidences. PCR with general and species-specific primers was used to establish that the predominant species associated with tomato curly top disease were Beet mild curly top virus (BMCTV) and, to a lesser extent, Beet severe curly top virus (BSCTV). The incidence, relative amount, and species of curly top virus in leafhoppers, collected at monthly intervals by the California Department of Food and Agriculture (CDFA) personnel during 2003 to 2008, was also determined. The predominant species detected were BMCTV and BSCTV. The highest incidences of curly top in tomato fields were associated with high populations of leafhoppers (e.g., in 2003 when populations were two times greater than average) having high incidences and levels of curly top virus early in the growing seasons (e.g., March to May 2003 and 2008). Detection of curly top virus in leafhoppers early in the growing season was consistent with acquisition of virus from reservoir hosts in the foothills. However, continued detection of curly top virus in leafhoppers throughout the growing season and development of curly top in late-planted fresh market tomatoes were consistent with the presence of inoculum sources (e.g., weeds or crop plants) in the agricultural areas of the Central Valley. Geographical locations or "hotspots" having higher proportions of curly top virus-positive leafhoppers were identified, which may reveal areas having high concentrations of curly top virus reservoir hosts. The application of these molecular tools has provided new insight into curly top of tomato in California, and may lead to improved curly top management.