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. 2019 Apr;50(2):335-345.
doi: 10.1007/s42770-019-00045-x. Epub 2019 Feb 13.

Classification of the inoculant strain of cowpea UFLA03-84 and of other strains from soils of the Amazon region as Bradyrhizobium viridifuturi (symbiovar tropici)

Elaine Martins da Costa  1   2 , Teotonio Soares de Carvalho  1 , Amanda Azarias Guimarães  1 , Aniele Carolina Ribas Leão  3 , Leonardo Magalhães Cruz  3 , Valter Antonio de Baura  3 , Liesbeth Lebbe  4 , Anne Willems  4 , Fatima Maria de Souza Moreira  5
Affiliations

Classification of the inoculant strain of cowpea UFLA03-84 and of other strains from soils of the Amazon region as Bradyrhizobium viridifuturi (symbiovar tropici)

Elaine Martins da Costa et al. Braz J Microbiol. 2019 Apr.

Abstract

Cowpea (Vigna unguiculata L.) is a legume species that considerably benefits from inoculation with nitrogen fixing bacteria of the genus Bradyrhizobium. One of the strains recommended for inoculation in cowpea in Brazil is UFLA03-84 (Bradyrhizobium sp.). The aim of our study was to define the taxonomic position of the UFLA03-84 strain and of two other strains of Bradyrhizobium (UFLA03-144 and INPA237B), all belonging to the same phylogenetic group and isolated from soils of the Brazilian Amazon. Multilocus sequence analysis (MLSA) of the housekeeping genes atpD, gyrB, recA, and rpoB grouped (with similarity higher than 99%) the three strains with Bradyrhizobium viridifuturi SEMIA 690T. The analyses of average nucleotide identity and digital DNA-DNA hybridization supported classification of the group as Bradyrhizobium viridifuturi. The three strains exhibited similar behavior in relation to the most of the phenotypic characteristics evaluated. However, some characteristics exhibited variation, indicating phenotypic diversity within the species. Phylogenetic analysis of the nodC and nifH genes showed that the three strains are members of the same symbiovar (tropici) that contains type strains of Bradyrhizobium species coming from tropical soils (SEMIA 690TB. viridifuturi, CNPSo 1112TB. tropiciagri, CNPSo 2833TB. embrapense, and B. brasilense UFLA03-321T).

Keywords: Average nucleotide identity; Bradyrhizobium; Genome; Housekeeping genes; Vigna unguiculata L..

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Conflict of interest statement

The authors declare that they have no conflict of interest.

Figures

Fig. 1
Fig. 1
Maximum likelihood phylogeny based on 16S rRNA gene sequences (1180 bp) showing the relationships between strains UFLA03-84, UFLA03-144, and INPA237B (in bold) and type strains of Bradyrhizobium species. GenBank accession numbers are provided in parentheses
Fig. 2
Fig. 2
Maximum likelihood phylogeny based on partial concatenated sequences (1816 bp) of housekeeping genes (atpD, gyrB, recA, and rpoB) showing the relationships between strains UFLA03-84, UFLA03-144, and INPA237B (in bold) and type strains of the Bradyrhizobium species. GenBank accession numbers are provided in parentheses
Fig. 3
Fig. 3
Maximum likelihood phylogeny based on partial sequences of nodC (400 bp) gene showing the relationships between strains UFLA03-84, UFLA03-144, and INPA237B (in bold) and type strains of Bradyrhizobium species. GenBank accession numbers are provided in parentheses
Fig. 4
Fig. 4
Maximum likelihood phylogeny based on partial sequences of the nifH (177 bp) gene showing the relationships between strains UFLA03-84, and UFLA03-144 and INPA237B (in bold) and type strains of Bradyrhizobium species. GenBank accession numbers are provided in parenthesis
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