Renal haemodynamics during hyperchloraemia in the anaesthetized dog: effects of captopril

Abstract

1. An increase in plasma chloride concentration (PC1) decreases renal blood flow (RBF) and glomerular filtration rate (GFR) and inhibits the intrarenal release of renin and angiotensin II (AII). Captopril was administered to indomethacin-treated dogs to assess the role of AII independent of prostaglandins (PGs) in the haemodynamic response to hyperchloraemia. Studies were performed on kidneys that were denervated by autotransplantation. 2. Anaesthetized greyhounds received an intrarenal infusion of 0.616 M-sodium acetate, which was changed to 0.616 M-NaCl (hyperchloraemia). These infusions increased the plasma sodium and osmolality at the experimental kidney by 7-11% throughout but increased the PCl during the hypertonic NaCl infusions only (122 +/- 3 to 136 +/- 3 mM). 3. In vehicle-treated dogs (n = 8), hyperchloraemia reduced the GFR (1.4 +/- 0.1 to 1.0 +/- 0.1 ml min-1 kg-1; P less than 0.05) and the RBF (13.0 +/- 1.4 to 8.3 +/- 0.6 ml min-1 kg-1; P less than 0.01); these changes were reversible on return to the 0.616 M-sodium acetate infusion. Hyperchloraemia reduced the release of AII into renal lymph (2.5 +/- 0.9 to 1.2 +/- 0.4 pg min-1 kg-1; P less than 0.01). 4. Indomethacin (0.6 mg kg-1 and 0.2 mg kg-1 h-1 intrarenally; n = 4) blunted (P less than 0.05) the Cl--induced fall in RBF (10.4 +/- 1.1 to 8.2 +/- 0.6 ml min-1 kg-1) without changing significantly the fall in GFR or the release of AII into renal lymph.(ABSTRACT TRUNCATED AT 250 WORDS)