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Review
. 2018 Feb 27;6(Suppl):S15-S26.
doi: 10.1016/j.ijvsm.2018.02.003. eCollection 2018.

Advances in in vitro production of sheep embryos

Jie Zhu  1 , Adel R Moawad  2 , Chun-Yu Wang  1 , Hui-Feng Li  1 , Jing-Yu Ren  1 , Yan-Feng Dai  1
Affiliations
Review

Advances in in vitro production of sheep embryos

Jie Zhu et al. Int J Vet Sci Med. .

Abstract

Sheep is an important livestock in the world providing meat, milk and wool for human beings. With increasing human population, the worldwide needs of production of sheep have elevated. To meet the needs, the assistant reproductive technology including ovine in vitro embryo production (ovine IVP) is urgently required to enhance the effective production of sheep in the world. To learn the status of ovine IVP, we collected some publications related to ovine IVP through PubMed and analyzed the progress in ovine IVP made in the last five years (2012-2017). We made comparisons of these data and found that the recent advances in ovine IVP has been made slowly comparable to that of ovine IVP two decades ago. Therefore, we suggested two strategies or approaches to tackle the main problems in ovine IVP and expect that the efficiency of ovine IVP could be improved significantly when the approaches would be implemented.

Keywords: Embryos; IVF; IVM; IVP; Oocyte; Sheep.

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Figures

Fig. 1
Fig. 1
Production of sheep in the world (dated from 2000 to 2014 cited at online from UN FAOSTAT-DATA 2017).
Fig. 2
Fig. 2
Curves of sheep oocyte in vitro maturation. The black line shows a normal curve of ovine oocyte IVM during IVM based on observation at our laboratory. The red line shows an ideal curve of ovine oocyte IVM presumably during maturation after synchronization of oocytes at the beginning of in vitro maturation. Maturation rate: the number of MII oocytes/the number of cultured oocytes x100% (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
Fig. 3
Fig. 3
Fertilization rate of in vitro matured sheep oocytes The black line shows a curve of fertilization rate of normal IVM oocytes;The red line shows presumably an ideal curve of fertilization of IVM oocytes with extended fertile span treated by an inhibitor. The fertilization rate (%) is defined with the number of cleaved oocytes/the number of cultured oocytes ×100%. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
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