Numerical Study of Enhancement of Positive Dielectrophoresis Particle Trapping in Electrode-Multilayered Microfluidic Device

Abstract

Enhancement of positive dielectrophoresis (pDEP) particle trapping by a co-occurring fluid flow under an ac electric field in an electrode-multilayered microfluidic device is investigated by three-dimensional particle-fluid flow simulations. The particle motion near one cross section of the microfluidic device is simulated under a zero flow condition by the Eulerian-Lagrangian method incorporating the ac electrothermal effect, thermal buoyancy, and dielectrophoresis. The mean trapping rate under the steady state R_m is evaluated from the simulated number of trapped particles N_trap for 54 cases with four parameters: electrode excitation pattern, medium conductivity σ, applied voltage ϕ_e, and the real part of the Clausius-Mossotti factor Re[K(ω)]. The simulated pDEP velocity in the upper part of the flow channel is validated by an experiment using cell suspension and is fitted so that the non-dimensional velocity error is within 15% of a typical velocity of pDEP. The mean trapping rate R_m is greatly increased by the fluid flow only in the high conductivity and high voltage cases. Regardless of the electrode excitation pattern, R_m increased almost proportionally to the inflow rate into the capture region, where the pDEP force is effective. From a fitted equation of the results, the increase of R_m when Re[K(ω)] = 0.1 to 0.5 is found to be about 20% to 30% of the number of particles transported into the capture regions. The results quantify the enhancement of pDEP trapping by the fluid flow occurring under practical conditions in the device.